Distinct contributions of meprins to skin regeneration after injury - Meprin α a physiological processer of pro-collagen VII.
ALP, astacin-like proteinase
BSA, bovine serum albumine
BTP, BMP-1/tolloid-like proteinase
DAPI, 4′-,6-diamidino-2-phenylindole
DEJ, dermal epidermal junction
DMEM, Dulbecco’s modified Eagle’s medium
Dystrophic epidermolysis bullosa
ECM, extracellular matrix
Extracellular matrix
FA, formic acid
FBS, fetal bovine serum
Fibrosis
Inflammation
NC, non-collagenous
PBS, phosphate-buffered saline
TBS, tris-buffered saline
WT, wild type
Wound healing
qPCR, quantitative polymerase chain reaction
αSMA, α-smooth muscle actin
Journal
Matrix biology plus
ISSN: 2590-0285
Titre abrégé: Matrix Biol Plus
Pays: Netherlands
ID NLM: 101775320
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
12
03
2021
revised:
28
04
2021
accepted:
03
05
2021
entrez:
26
8
2021
pubmed:
27
8
2021
medline:
27
8
2021
Statut:
epublish
Résumé
Astacin-like proteinases (ALPs) are regulators of tissue and extracellular matrix (ECM) homeostasis. They convey this property through their ability to convert ECM protein pro-forms to functional mature proteins and by regulating the bioavailability of growth factors that stimulate ECM synthesis. The most studied ALPs in this context are the BMP-1/tolloid-like proteinases. The other subclass of ALPs in vertebrates - the meprins, comprised of meprin α and meprin β - are emerging as regulators of tissue and ECM homeostasis but have so far been only limitedly investigated. Here, we functionally assessed the roles of meprins in skin wound healing using mice genetically deficient in one or both meprins. Meprin deficiency did not change the course of macroscopic wound closure. However, subtle but distinct contributions of meprins to the healing process and dermal homeostasis were observed. Loss of both meprins delayed re-epithelialization and reduced macrophage infiltration. Abnormal dermal healing and ECM regeneration was observed in meprin deficient wounds. Our analyses also revealed meprin α as one proteinase responsible for maturation of pro-collagen VII to anchoring fibril-forming-competent collagen VII
Identifiants
pubmed: 34435182
doi: 10.1016/j.mbplus.2021.100065
pii: S2590-0285(21)00009-0
pmc: PMC8377016
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100065Informations de copyright
© 2021 The Authors.
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