Simultaneous genotyping of snails and infecting trematode parasites using high-throughput amplicon sequencing.
Gastropoda
co-infections
community ecology
diagnostics
high-throughput amplicon sequencing
schistosomiasis
Journal
Molecular ecology resources
ISSN: 1755-0998
Titre abrégé: Mol Ecol Resour
Pays: England
ID NLM: 101465604
Informations de publication
Date de publication:
Feb 2022
Feb 2022
Historique:
revised:
19
07
2021
received:
25
09
2020
accepted:
18
08
2021
pubmed:
27
8
2021
medline:
6
1
2022
entrez:
26
8
2021
Statut:
ppublish
Résumé
Several methodological issues currently hamper the study of entire trematode communities within populations of their intermediate snail hosts. Here we develop a new workflow using high-throughput amplicon sequencing to simultaneously genotype snail hosts and their infecting trematode parasites. We designed primers to amplify four snail and five trematode markers in a single multiplex PCR. While also applicable to other genera, we focused on medically and economically important snail genera within the superorder Hygrophila and targeted a broad taxonomic range of parasites within the class Trematoda. We tested the workflow using 417 Biomphalaria glabrata specimens experimentally infected with Schistosoma rodhaini, two strains of Schistosoma mansoni and combinations thereof. We evaluated the reliability of infection diagnostics, the robustness of the workflow, its specificity related to host and parasite identification, and the sensitivity to detect co-infections, immature infections and changes of parasite biomass during the infection process. Finally, we investigated its applicability in wild-caught snails of other genera naturally infected with a diverse range of trematodes. After stringent quality control the workflow allows the identification of snails to species level, and of trematodes to taxonomic levels ranging from family to strain. It is sensitive to detect immature infections and changes in parasite biomass described in previous experimental studies. Co-infections were successfully identified, opening the possibility to examine parasite-parasite interactions such as interspecific competition. Together, these results demonstrate that our workflow provides a powerful tool to analyse the processes shaping trematode communities within natural snail populations.
Identifiants
pubmed: 34435445
doi: 10.1111/1755-0998.13492
doi:
Banques de données
GENBANK
['MZ546824', 'MZ546834', 'MZ540237', 'MZ540247', 'MZ600069', 'MZ600118', 'MZ546798', 'MZ546803', 'MZ567152', 'MZ567158', 'MZ558220', 'MZ558244', 'MZ600119', 'MZ600142', 'MZ546812', 'MZ546823', 'MZ546804', 'MZ546811']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
567-586Subventions
Organisme : Laboratoire d'Excellence (LabEx) TULIP
ID : ANR-10-LABX-41
Organisme : ANR HySWARM
ID : ANR-18-CE35-0001
Organisme : Fonds Européens de Développement Régional
Organisme : BRAIN-be
ID : Pioneer Project BR/165/PI/TRAIL
Organisme : Fonds Wetenschappelijk Onderzoek
ID : Ph.D. Fellowship 11C5219N
Organisme : Agence Nationale de la Recherche
ID : ANR-17-CE02-0015
Informations de copyright
© 2021 John Wiley & Sons Ltd.
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