All the Pups We Cannot See: Cannibalism Masks Perinatal Death in Laboratory Mouse Breeding but Infanticide Is Rare.

3Rs principle asynchrony breeding cage inspection cannibalistic behaviour husbandry practices mouse welfare neonatal mortality pup-counting method social environment

Journal

Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614

Informations de publication

Date de publication:
06 Aug 2021
Historique:
received: 26 06 2021
revised: 29 07 2021
accepted: 03 08 2021
entrez: 27 8 2021
pubmed: 28 8 2021
medline: 28 8 2021
Statut: epublish

Résumé

Perinatal mortality is a major issue in laboratory mouse breeding. We compared a counting method using daily checks (DAILY_CHECK) with a method combining daily checks with detailed video analyses to detect cannibalisms (VIDEO_TRACK) for estimating the number of C57BL/6 pups that were born, that died and that were weaned in 193 litters from trios with (TRIO-OVERLAP) or without (TRIO-NO_OVERLAP) the presence of another litter. Linear mixed models were used at litter level. To understand whether cannibalism was associated with active killing (infanticide), we analysed VIDEO_TRACK recordings of 109 litters from TRIO-OVERLAP, TRIO-NO_OVERLAP or SOLO (single dams). We used Kaplan-Meier method and logistic regression at pup level. For DAILY_CHECK, the mean litter size was 35% smaller than for VIDEO_TRACK (

Identifiants

pubmed: 34438784
pii: ani11082327
doi: 10.3390/ani11082327
pmc: PMC8388445
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : European Regional Development Fund
ID : POCI-01-0145-FEDER-016591
Organisme : Fundação para a Ciência e a Tecnologia
ID : PTDC/CVT-WEL/1202/2014

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Auteurs

Sophie Brajon (S)

Laboratory Animal Science, IBMC-Instituto de Biologia Molecular e Celular, and i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal.
Babraham Institute, Babraham, Cambridge CB22 3AT, UK.

Gabriela Munhoz Morello (GM)

Laboratory Animal Science, IBMC-Instituto de Biologia Molecular e Celular, and i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal.

Sara Capas-Peneda (S)

Laboratory Animal Science, IBMC-Instituto de Biologia Molecular e Celular, and i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal.
ICBAS School of Medicine and Biomedical Sciences, University of Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal.
The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.

Jan Hultgren (J)

Department of Animal Environment and Health, Swedish University of Agricultural Sciences, 53223 Skara, Sweden.

Colin Gilbert (C)

Babraham Institute, Babraham, Cambridge CB22 3AT, UK.

Anna Olsson (A)

Laboratory Animal Science, IBMC-Instituto de Biologia Molecular e Celular, and i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal.

Classifications MeSH