Prevention of Teratogenesis in Pregnancies of Obese Rats by Vitamin E Supplementation.
embryo malformation
glutathione
obesity
oxidative stress
teratogenesis
vitamin E
Journal
Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
Titre abrégé: Antioxidants (Basel)
Pays: Switzerland
ID NLM: 101668981
Informations de publication
Date de publication:
23 Jul 2021
23 Jul 2021
Historique:
received:
28
05
2021
revised:
12
07
2021
accepted:
15
07
2021
entrez:
27
8
2021
pubmed:
28
8
2021
medline:
28
8
2021
Statut:
epublish
Résumé
Congenital malformations are a common adverse outcome in pregnancies complicated by pregestational obesity, although the underlying mechanisms are still unrevealed. Our aim was to study the effect of oxidative stress in obesity-induced teratogenesis. Wistar rats were fed a high-fat diet for 13 weeks, with (OE group) or without (O group) vitamin E supplementation. Then, rats were mated and sacrificed at day 11.5 of gestation. Embryos from O dams presented a 25.9 ± 3.5% rate of malformations (vs. 8.7 ± 3.4% in C rats), which was reduced in the OE group (11.5 ± 2.3%). Pregestational obesity induced hepatic protein and DNA oxidation and a decline in antioxidant enzymes. Importantly, glutathione content was also decreased, limiting the availability of this antioxidant in the embryos. Vitamin E supplementation efficiently maintained glutathione levels in the obese mothers, which could be used in their embryos to prevent oxidation-induced malformations. To test the effect of decreasing glutathione levels alone in a cell culture model of neuroepithelium, murine embryonic stem cells (ESC) were induced to form neuronal precursors and glutathione synthesis was inhibited with the gamma-glutamylcysteine synthesis inhibitor, buthionine sulfoximine (BSO). BSO inhibited the expression of
Identifiants
pubmed: 34439421
pii: antiox10081173
doi: 10.3390/antiox10081173
pmc: PMC8389020
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NIH HHS
ID : P30DK036836
Pays : United States
Organisme : Fundación Universitaria CEU San Pablo - Banco Santander
ID : FUSP-PPC-19-C91B8F34
Organisme : Fundación Universitaria CEU San Pablo - Banco Santander
ID : FUSP-BS-PPC USP02/2017
Organisme : NIDDK NIH HHS
ID : P30 DK036836
Pays : United States
Organisme : NIH HHS
ID : R01DK104649
Pays : United States
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