Impact of the Epigenetically Regulated Hoxa-5 Gene in Neural Differentiation from Human Adipose-Derived Stem Cells.
CRISPR/dCas9
Hoxa-5
epigenetic changes
mesenchymal stem cells
neuronal differentiation
Journal
Biology
ISSN: 2079-7737
Titre abrégé: Biology (Basel)
Pays: Switzerland
ID NLM: 101587988
Informations de publication
Date de publication:
19 Aug 2021
19 Aug 2021
Historique:
received:
15
07
2021
revised:
11
08
2021
accepted:
16
08
2021
entrez:
27
8
2021
pubmed:
28
8
2021
medline:
28
8
2021
Statut:
epublish
Résumé
Human adipose-derived mesenchymal stem cells (hASCs) may be used in some nervous system pathologies, although obtaining an adequate degree of neuronal differentiation is an important barrier to their applicability. This requires a deep understanding of the expression and epigenetic changes of the most important genes involved in their differentiation. We used hASCs from human lipoaspirates to induce neuronal-like cells through three protocols (Neu1, 2, and 3), determined the degree of neuronal differentiation using specific biomarkers in culture cells and neurospheres, and analyzed epigenetic changes of genes involved in this differentiation. Furthermore, we selected the
Identifiants
pubmed: 34440035
pii: biology10080802
doi: 10.3390/biology10080802
pmc: PMC8389620
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Fundació La Marató TV3
ID : 111430/31
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