Menaquinone 4 Reduces Bone Loss in Ovariectomized Mice through Dual Regulation of Bone Remodeling.
Animals
Body Weight
/ drug effects
Bone Density
/ drug effects
Bone Remodeling
/ drug effects
Bone Resorption
/ genetics
Bone and Bones
/ metabolism
Disease Models, Animal
Female
Gene Expression
Humans
Mice
Mice, Inbred C57BL
Organ Size
/ drug effects
Osteogenesis
/ genetics
Osteoporosis, Postmenopausal
/ prevention & control
Ovariectomy
Uterus
/ drug effects
Vitamin K 2
/ administration & dosage
hematoxylin-eosin staining
mRNA expression
menaquinone 4
micro-CT
ovariectomy
Journal
Nutrients
ISSN: 2072-6643
Titre abrégé: Nutrients
Pays: Switzerland
ID NLM: 101521595
Informations de publication
Date de publication:
27 Jul 2021
27 Jul 2021
Historique:
received:
01
07
2021
revised:
22
07
2021
accepted:
26
07
2021
entrez:
27
8
2021
pubmed:
28
8
2021
medline:
18
9
2021
Statut:
epublish
Résumé
Epidemiologic studies showed that higher vitamin K (VK) consumption correlates with a reduced risk of osteoporosis, yet the dispute remains about whether VK is effective in improving bone mineral density (BMD). We sought to discover the anti-osteoporotic effect of menaquinone-4 (MK-4) and evaluate the expression of critical genes related to bone formation and bone resorption pathways in the body. Fifty female C57BL/6 mice (aged 13 weeks) were randomly arranged to a sham-operated group (SHAM, treated with corn oil) and four ovariectomized groups that were administered corn oil (OVX group), estradiol valerate (EV, 2 mg/kg body weight as the positive control), low or high doses of VK (LVK and HVK; 20 and 40 mg MK-4/kg body weight, respectively) by gavage every other day for 12 weeks. Body and uterine weight, serum biochemical indicators, bone microarchitecture, hematoxylin-eosin (HE) staining, and the mRNA expression of critical genes related to bone formation and bone resorption pathways were assessed. Either dose of MK-4 supplementation increased the alkaline phosphatase (ALP), decreased the undercarboxylated osteocalcin (ucOC) and tartrate-resistant acid phosphatase (TRACP,
Identifiants
pubmed: 34444729
pii: nu13082570
doi: 10.3390/nu13082570
pmc: PMC8398915
pii:
doi:
Substances chimiques
Vitamin K 2
11032-49-8
menatetrenone
27Y876D139
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Natural Science Foundation of China
ID : 32072740
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