Axonal Injuries Cast Long Shadows: Long Term Glial Activation in Injured and Contralateral Retinas after Unilateral Axotomy.
M1
M2
Müller cells
astrocytes
bilateral response
inflammation
microglial cells
optic nerve crush
retina
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
07 Aug 2021
07 Aug 2021
Historique:
received:
06
07
2021
revised:
29
07
2021
accepted:
04
08
2021
entrez:
27
8
2021
pubmed:
28
8
2021
medline:
15
9
2021
Statut:
epublish
Résumé
To analyze the course of microglial and macroglial activation in injured and contralateral retinas after unilateral optic nerve crush (ONC). The left optic nerve of adult pigmented C57Bl/6 female mice was intraorbitally crushed and injured, and contralateral retinas were analyzed from 1 to 45 days post-lesion (dpl) in cross-sections and flat mounts. As controls, intact retinas were studied. Iba1 After ONC, MC density increased significantly from 5 to 21 dpl in the inner layers of injured retinas, remaining within intact values in the contralateral ones. However, in both retinas there was a significant and long-lasting increase of CD68 Unilateral ONC triggers a bilateral and persistent activation of MCs and an opposed response of M2 MCs between both retinas. Macroglial hypertrophy is transient.
Sections du résumé
BACKGROUND
BACKGROUND
To analyze the course of microglial and macroglial activation in injured and contralateral retinas after unilateral optic nerve crush (ONC).
METHODS
METHODS
The left optic nerve of adult pigmented C57Bl/6 female mice was intraorbitally crushed and injured, and contralateral retinas were analyzed from 1 to 45 days post-lesion (dpl) in cross-sections and flat mounts. As controls, intact retinas were studied. Iba1
RESULTS
RESULTS
After ONC, MC density increased significantly from 5 to 21 dpl in the inner layers of injured retinas, remaining within intact values in the contralateral ones. However, in both retinas there was a significant and long-lasting increase of CD68
CONCLUSIONS
CONCLUSIONS
Unilateral ONC triggers a bilateral and persistent activation of MCs and an opposed response of M2 MCs between both retinas. Macroglial hypertrophy is transient.
Identifiants
pubmed: 34445225
pii: ijms22168517
doi: 10.3390/ijms22168517
pmc: PMC8395228
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Instituto de Salud Carlos III
ID : PI19/00071
Organisme : Instituto de Salud Carlos III
ID : RD16/0008/0026
Organisme : Ministerio de Economía y Competitividad
ID : PID2019-106498GB-I00
Organisme : Fundación Séneca
ID : 19881/GERM/15
Références
Front Syst Neurosci. 2019 Aug 27;13:37
pubmed: 31507384
Sci Rep. 2013 Oct 30;3:3072
pubmed: 24170042
Neurosci Lett. 1991 Jun 10;127(1):108-12
pubmed: 1881605
Cell Discov. 2018 Feb 27;4:9
pubmed: 29507754
Prog Retin Eye Res. 2006 Jul;25(4):397-424
pubmed: 16839797
Prog Retin Eye Res. 2014 Nov;43:17-75
pubmed: 25038518
Brain Behav Immun. 2013 Aug;32:70-85
pubmed: 23454862
Science. 2010 Nov 5;330(6005):841-5
pubmed: 20966214
Ocul Immunol Inflamm. 2002 Mar;10(1):27-39
pubmed: 12461701
Mediators Inflamm. 2015;2015:673090
pubmed: 25873768
Int J Mol Sci. 2019 Nov 15;20(22):
pubmed: 31731684
Nat Neurosci. 2016 Jul 26;19(8):987-91
pubmed: 27459405
Br J Pharmacol. 2016 Feb;173(4):649-65
pubmed: 25800044
PLoS One. 2012;7(2):e30763
pubmed: 22363486
Front Neuroanat. 2017 Sep 05;11:77
pubmed: 28928639
Mol Neurobiol. 2016 Mar;53(2):1181-1194
pubmed: 25598354
Neural Regen Res. 2019 Jul;14(7):1186-1190
pubmed: 30804243
Front Immunol. 2019 Aug 20;10:1975
pubmed: 31481963
Invest Ophthalmol Vis Sci. 2009 Aug;50(8):3860-8
pubmed: 19264888
J Neuroinflammation. 2014 Jul 26;11:133
pubmed: 25064005
Alzheimers Res Ther. 2015 Aug 19;7(1):56
pubmed: 26286145
Prog Retin Eye Res. 2016 Mar;51:1-40
pubmed: 26113209
Exp Eye Res. 1992 Jul;55(1):101-17
pubmed: 1383017
Semin Cell Dev Biol. 2019 Oct;94:96-103
pubmed: 30826549
J Neurobiol. 1993 Jan;24(1):23-36
pubmed: 8419522
Neural Regen Res. 2021 Nov;16(11):2125-2131
pubmed: 33818483
J Neuroinflammation. 2012 May 14;9:92
pubmed: 22583833
Nat Neurosci. 2021 Mar;24(3):312-325
pubmed: 33589835
Invest Ophthalmol Vis Sci. 2013 Feb 01;54(2):974-85
pubmed: 23307961
Rheumatology (Oxford). 2003 Nov;42(11):1279-86
pubmed: 12867588
J Neurosci Res. 2013 Sep;91(9):1143-51
pubmed: 23686747
Sci Rep. 2020 Mar 17;10(1):4890
pubmed: 32184450
Front Aging Neurosci. 2021 Apr 06;13:651973
pubmed: 33889084
Brain Res Mol Brain Res. 1998 Jun 1;57(1):1-9
pubmed: 9630473
Inflamm Res. 2021 Feb;70(2):183-192
pubmed: 33386422
Exp Eye Res. 2011 May;92(5):377-87
pubmed: 21354138
Neurochem Res. 2020 May;45(5):1072-1085
pubmed: 32052258
J Neuroinflammation. 2017 Nov 09;14(1):218
pubmed: 29121969
Invest Ophthalmol Vis Sci. 2020 Mar 9;61(3):47
pubmed: 32232352
J Comp Neurol. 2007 Apr 20;501(6):866-78
pubmed: 17311318
Front Neurosci. 2017 Apr 26;11:235
pubmed: 28491019
J Neurocytol. 1992 Jul;21(7):530-44
pubmed: 1500949
Exp Eye Res. 2018 May;170:40-50
pubmed: 29452106
PLoS One. 2013 Dec 18;8(12):e83733
pubmed: 24367610
Front Neurosci. 2019 Sep 04;13:925
pubmed: 31551688
Invest Ophthalmol Vis Sci. 2010 Nov;51(11):5690-6
pubmed: 20538983