Endogenous Enzymatic Activity in Dentin Treated with a Chitosan Primer.
chitosan
collagen cross-linker
dentin
matrix metalloproteinases
zymography
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
17 Aug 2021
17 Aug 2021
Historique:
received:
16
07
2021
revised:
11
08
2021
accepted:
11
08
2021
entrez:
27
8
2021
pubmed:
28
8
2021
medline:
14
9
2021
Statut:
epublish
Résumé
The aim of this study was to evaluate the effect of different concentrations of chitosan polymer on dentinal enzymatic activity by means of gelatin and in situ zymography. Human dentin was frozen and ground in a miller. Dentin powder aliquots were demineralized with phosphoric acid and treated with three different concentrations of lyophilized chitosan polymer (1, 0.5 and 0.1 wt%) dissolved in distilled water. Dentin proteins were extracted from each experimental group and electrophoresed under non-reducing conditions in 10% SDS-PAGE containing fluorescein-labeled gelatin. After 48 h in the incubation buffer at 37 °C, proteolytic activity was registered under long-wave UV light scanner and quantified by using Image J software. Furthermore, additional teeth (
Identifiants
pubmed: 34445554
pii: ijms22168852
doi: 10.3390/ijms22168852
pmc: PMC8396363
pii:
doi:
Substances chimiques
Chelating Agents
0
Chitosan
9012-76-4
Matrix Metalloproteinases
EC 3.4.24.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Federation of Conservative Dentistry
ID : EFCD Scientific Foundation scholarship for Eugenia Baena
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