Modulation of the mTOR pathway plays a central role in dendritic cell functions after Echinococcus granulosus antigen recognition.
Animals
Antigens, Helminth
/ immunology
Autophagosomes
/ immunology
Cell Proliferation
Cells, Cultured
Cytokines
/ genetics
Dendritic Cells
/ cytology
Echinococcosis
/ immunology
Echinococcus granulosus
/ immunology
Female
Flow Cytometry
Mice
Microscopy, Confocal
Signal Transduction
/ immunology
T-Lymphocytes
/ immunology
TOR Serine-Threonine Kinases
/ immunology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
26 08 2021
26 08 2021
Historique:
received:
28
07
2020
accepted:
09
07
2021
entrez:
27
8
2021
pubmed:
28
8
2021
medline:
9
11
2021
Statut:
epublish
Résumé
Immune evasion is a hallmark of persistent echinococcal infection, comprising modulation of innate immune cells and antigen-specific T cell responses. However, recognition of Echinococcus granulosus by dendritic cells (DCs) is a key determinant of the host's response to this parasite. Given that mTOR signaling pathway has been described as a regulator linking metabolism and immune function in DCs, we reported for the first time in these cells, global translation levels, antigen uptake, phenotype, cytokine transcriptional levels, and splenocyte priming activity upon recognition of the hydatid fluid (HF) and the highly glycosylated laminar layer (LL). We found that LL induced a slight up-regulation of CD86 and MHC II in DCs and also stimulated the production of IL-6 and TNF-α. By contrast, HF did not increase the expression of any co-stimulatory molecules, but also down-modulated CD40 and stimulated the expression of the anti-inflammatory cytokine IL-10. Both parasitic antigens promoted protein synthesis through mTOR activation. The use of rapamycin decreased the expression of the cytokines tested, empowered the down-modulation of CD40 and also reduced splenocyte proliferation. Finally, we showed that E. granulosus antigens increase the amounts of LC3-positive structures in DCs which play critical roles in the presentation of these antigens to T cells.
Identifiants
pubmed: 34446757
doi: 10.1038/s41598-021-96435-z
pii: 10.1038/s41598-021-96435-z
pmc: PMC8390662
doi:
Substances chimiques
Antigens, Helminth
0
Cytokines
0
TOR Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17238Informations de copyright
© 2021. The Author(s).
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