Germline-Dependent Antibody Paratope States and Pairing Specific V
VH-VL pairings
antibodies
backbone vs. sidechain flexibility
germlines
paratope states in solution
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
03
03
2021
accepted:
24
06
2021
entrez:
27
8
2021
pubmed:
28
8
2021
medline:
25
2
2023
Statut:
epublish
Résumé
Antibodies have emerged as one of the fastest growing classes of biotherapeutic proteins. To improve the rational design of antibodies, we investigate the conformational diversity of 16 different germline combinations, which are composed of 4 different kappa light chains paired with 4 different heavy chains. In this study, we systematically show that different heavy and light chain pairings strongly influence the paratope, interdomain interaction patterns and the relative V
Identifiants
pubmed: 34447370
doi: 10.3389/fimmu.2021.675655
pmc: PMC8382685
doi:
Substances chimiques
Complementarity Determining Regions
0
Immunoglobulin Heavy Chains
0
Immunoglobulin Light Chains
0
Immunoglobulin Variable Region
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
675655Informations de copyright
Copyright © 2021 Fernández-Quintero, Kroell, Bacher, Loeffler, Quoika, Georges, Bujotzek, Kettenberger and Liedl.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Exp Med. 2004 Jul 19;200(2):191-9
pubmed: 15263026
J Mol Biol. 1992 Mar 20;224(2):487-99
pubmed: 1560463
Protein Eng Des Sel. 2009 Mar;22(3):121-34
pubmed: 19136675
Bioinformatics. 2016 Jan 15;32(2):298-300
pubmed: 26424857
J Chem Theory Comput. 2015 Nov 10;11(11):5525-42
pubmed: 26574340
Protein Sci. 2003 Oct;12(10):2125-31
pubmed: 14500870
Nature. 1989 Dec 21-28;342(6252):877-83
pubmed: 2687698
Front Immunol. 2021 Mar 02;12:630034
pubmed: 33737932
MAbs. 2018 Feb/Mar;10(2):269-277
pubmed: 29283291
Nucleic Acids Res. 2015 Jan;43(Database issue):D432-8
pubmed: 25392411
J Mol Biol. 2011 Feb 18;406(2):228-56
pubmed: 21035459
Immunity. 2020 Dec 15;53(6):1230-1244.e5
pubmed: 33096040
J Mol Biol. 1996 Nov 15;263(5):800-15
pubmed: 8947577
Br J Cancer. 2004 May 4;90(9):1863-70
pubmed: 15150594
Proteins. 2020 Nov;88(11):1447-1457
pubmed: 32526069
Proteins. 2015 Apr;83(4):681-95
pubmed: 25641019
Bioinformatics. 2017 May 1;33(9):1346-1353
pubmed: 28453681
MAbs. 2020 Jan-Dec;12(1):1744328
pubmed: 32264741
J Mol Biol. 2005 Apr 8;347(4):773-89
pubmed: 15769469
Protein Eng Des Sel. 2012 Oct;25(10):523-9
pubmed: 22802295
Bioinformatics. 2013 Apr 1;29(7):845-54
pubmed: 23407358
J Immunol. 1998 May 15;160(10):4762-7
pubmed: 9590222
J Chem Theory Comput. 2014 Jan 14;10(1):381-90
pubmed: 26579917
Biophys J. 2000 Aug;79(2):614-28
pubmed: 10919996
Protein Eng Des Sel. 2019 Dec 31;32(9):411-422
pubmed: 32129452
J Chem Theory Comput. 2015 Aug 11;11(8):3696-713
pubmed: 26574453
J Mol Biol. 1997 Nov 7;273(4):927-48
pubmed: 9367782
Front Mol Biosci. 2020 Nov 24;7:609088
pubmed: 33330636
Proteins. 2020 Jul;88(7):830-839
pubmed: 31904133
MAbs. 2016 Aug-Sep;8(6):1045-63
pubmed: 27210805
J Mol Biol. 1963 Jul;7:95-9
pubmed: 13990617
Proteins. 2009 Apr;75(1):187-205
pubmed: 18814299
Protein Eng. 1999 Sep;12(9):713-20
pubmed: 10506280
MAbs. 2020 Jan-Dec;12(1):1703531
pubmed: 31847708
J Chem Theory Comput. 2013 Sep 10;9(9):3878-88
pubmed: 26592383
Front Immunol. 2019 Nov 19;10:2652
pubmed: 31803187
Proteins. 2017 Jul;85(7):1311-1318
pubmed: 28342222
J Chem Theory Comput. 2007 Nov;3(6):2312-34
pubmed: 26636222
J Mol Biol. 1985 Dec 5;186(3):651-63
pubmed: 4093982
Front Mol Biosci. 2020 Aug 07;7:182
pubmed: 32850970
Biophys J. 2021 Jan 5;120(1):143-157
pubmed: 33220303
J Chem Theory Comput. 2013 Jul 9;9(7):3084-95
pubmed: 26583988
J Allergy Clin Immunol. 2010 Feb;125(2 Suppl 2):S41-52
pubmed: 20176268
J Clin Invest. 1996 Nov 15;98(10):2235-43
pubmed: 8941639
MAbs. 2019 Jan;11(1):58-74
pubmed: 30212263
Protein Eng Des Sel. 2019 Dec 31;32(11):513-523
pubmed: 32719844
Comput Struct Biotechnol J. 2017 Feb 01;15:222-231
pubmed: 28228926
MAbs. 2020 Jan-Dec;12(1):1840005
pubmed: 33180672
J Mol Biol. 1998 May 1;278(2):301-6
pubmed: 9571052
J Phys Chem B. 2018 May 31;122(21):5508-5514
pubmed: 29338243
J Biol Chem. 2008 Jan 11;283(2):1156-66
pubmed: 17947238
Protein Eng Des Sel. 2013 Oct;26(10):611-20
pubmed: 23708320
Commun Biol. 2020 Oct 20;3(1):589
pubmed: 33082531
Front Immunol. 2020 Jul 08;11:1440
pubmed: 32733478
Phys Rev Lett. 2008 Jan 18;100(2):020603
pubmed: 18232845
J Mol Biol. 2006 Apr 14;357(5):1566-74
pubmed: 16497332
J Mol Biol. 1990 Sep 5;215(1):175-82
pubmed: 2118959
MAbs. 2019 Aug/Sep;11(6):1077-1088
pubmed: 31148507
Blood. 2011 Jul 14;118(2):358-67
pubmed: 21444918
J Comput Chem. 2011 Jun;32(8):1743-52
pubmed: 21374633
J Chem Inf Model. 2021 Apr 26;61(4):1533-1538
pubmed: 33719418
J Phys Chem B. 2013 Oct 10;117(40):12286-95
pubmed: 24028495
Front Immunol. 2019 Jan 07;9:3065
pubmed: 30666252
MAbs. 2016;8(2):288-305
pubmed: 26637054
Nucleic Acids Res. 2009 Jan;37(Database issue):D1006-12
pubmed: 18978023
Curr Opin Struct Biol. 2014 Apr;25:135-44
pubmed: 24836551
Sci Rep. 2020 Mar 11;10(1):4472
pubmed: 32161287
J Mol Biol. 1998 Jan 16;275(2):269-94
pubmed: 9466909
Front Immunol. 2016 Sep 27;7:388
pubmed: 27729912
Nat Protoc. 2017 Feb;12(2):401-416
pubmed: 28125104