Development of ex situ normothermic reperfusion as an innovative method to assess pancreases after preservation.
normothermic reperfusion
organ assessment
oxygenated hypothermic machine perfusion
pancreas preservation
pancreas transplantation
porcine model
Journal
Transplant international : official journal of the European Society for Organ Transplantation
ISSN: 1432-2277
Titre abrégé: Transpl Int
Pays: Switzerland
ID NLM: 8908516
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
28
06
2021
accepted:
07
07
2021
entrez:
27
8
2021
pubmed:
28
8
2021
medline:
21
10
2021
Statut:
ppublish
Résumé
Static cold storage (SCS) is the standard method for pancreas preservation prior to transplantation; however, it does not permit organ assessment. Normothermic reperfusion (NR) is utilized clinically for other organs to assess viability. Our aim was to develop NR using normothermic machine perfusion technique to simulate reperfusion at the time of transplantation, enabling evaluation of oxygenated hypothermic machine perfusion (HMPO2) as a newer strategy to optimize pancreas preservation. 13 porcine pancreases procured after circulatory death were divided into 3 groups: 4 pancreases preserved using SCS, and 2 groups preserved by HMPO2 (n = 4 and n = 5, differing by type of preservation solution). Duration of perfusion or cold storage was 6 hours before the 1-hour assessment using NR. Outcome measures were perfusion characteristics, biochemistry and change in tissue water mass as oedema assessment. During NR, the HMPO2 groups demonstrated better perfusion characteristics, normal macroscopic appearances, decreased water mass and one HMPO2 group demonstrated a response to glucose stimulation. Conversely, the SCS group showed an increased water mass and developed early macroscopic appearances of oedema, interstitial haemorrhage and minimal portal outflow. This study suggests that ex situ assessment of pancreases by NR is promising, and that HMPO2 may be better than SCS.
Substances chimiques
Pancrelipase
53608-75-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1630-1642Subventions
Organisme : Institut Georges Lopez, France
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021 The Authors. Transplant International published by John Wiley & Sons Ltd on behalf of Steunstichting ESOT.
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