Development of HRM real-time PCR for assemblage characterization of Giardia lamblia.
Dog
Giardia, Assemblage
HRM real-time PCR
Humans
PCR/sequencing
Journal
Acta tropica
ISSN: 1873-6254
Titre abrégé: Acta Trop
Pays: Netherlands
ID NLM: 0370374
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
16
01
2021
revised:
14
08
2021
accepted:
18
08
2021
pubmed:
28
8
2021
medline:
26
11
2021
entrez:
27
8
2021
Statut:
ppublish
Résumé
A total of 90 stool samples were collected from dogs, referred to a dog shelter and a veterinary clinic. In addition, 395 stool samples obtained from pet dog owners and shelter keepers, as well as individuals referred to a medical laboratory as controls, were collected in Shahryar district, Tehran, Iran. Stool samples were parasitologically examined and the positive G. lamblia isolates were tested with Nested-PCR/sequencing for the tpi, gdh, and bg genes, and HRM real-time PCR. Microscopical examination revealed 20 (22.2%) and 34 (8.6%) Giardia-positive samples from dogs and humans, respectively. Regarding HRM real-time PCR, the prevalence of assemblages A and B in humans was 55.8% and 14.7%, respectively. In addition, 14.7% of samples were mix assemblages. HRM real-time PCR detected most of microscopically-positive samples in comparison to PCR/sequencing in both humans and dogs. The high prevalence of assemblages A and B in dogs signified the importance of a same source for infection between dogs and humans.
Identifiants
pubmed: 34450062
pii: S0001-706X(21)00288-6
doi: 10.1016/j.actatropica.2021.106109
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106109Informations de copyright
Copyright © 2021. Published by Elsevier B.V.