Novel Circulating and Tissue Monocytes as Well as Macrophages in Pancreatitis and Recovery.
Animals
Biomarkers
/ blood
Cell Separation
Disease Models, Animal
Female
Flow Cytometry
Humans
Immunity, Innate
Immunophenotyping
Macrophages
/ immunology
Mice, Inbred BALB C
Monocytes
/ immunology
Pancreas
/ immunology
Pancreatitis
/ blood
Phenotype
Recovery of Function
Severity of Illness Index
Time Factors
Acute Pancreatitis
CyTOF
Macrophages
Monocytes
Severe Acute Pancreatitis
Journal
Gastroenterology
ISSN: 1528-0012
Titre abrégé: Gastroenterology
Pays: United States
ID NLM: 0374630
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
received:
08
02
2021
revised:
28
07
2021
accepted:
17
08
2021
pubmed:
28
8
2021
medline:
19
1
2022
entrez:
27
8
2021
Statut:
ppublish
Résumé
Acute pancreatitis (AP) is an inflammatory disease with mild to severe course that is associated with local and systemic complications and significant mortality. Uncovering inflammatory pathways that lead to progression and recovery will inform ways to monitor and/or develop effective therapies. We performed single-cell mass Cytometry by Time Of Flight (CyTOF) analysis to identify pancreatic and systemic inflammatory signals during mild AP (referred to as AP), severe AP (SAP), and recovery using 2 independent experimental models and blood from patients with AP and recurrent AP. Flow cytometric validation of monocytes subsets identified using CyTOF analysis was performed independently. Ly6C We identified several novel monocyte/macrophage subsets with unique phenotype and functional characteristics that are associated with AP, recovery, and SAP. Our findings highlight differential innate immune responses during AP progression and recovery that can be leveraged for future disease monitoring and targeting.
Sections du résumé
BACKGROUND AND AIMS
Acute pancreatitis (AP) is an inflammatory disease with mild to severe course that is associated with local and systemic complications and significant mortality. Uncovering inflammatory pathways that lead to progression and recovery will inform ways to monitor and/or develop effective therapies.
METHODS
We performed single-cell mass Cytometry by Time Of Flight (CyTOF) analysis to identify pancreatic and systemic inflammatory signals during mild AP (referred to as AP), severe AP (SAP), and recovery using 2 independent experimental models and blood from patients with AP and recurrent AP. Flow cytometric validation of monocytes subsets identified using CyTOF analysis was performed independently.
RESULTS
Ly6C
CONCLUSIONS
We identified several novel monocyte/macrophage subsets with unique phenotype and functional characteristics that are associated with AP, recovery, and SAP. Our findings highlight differential innate immune responses during AP progression and recovery that can be leveraged for future disease monitoring and targeting.
Identifiants
pubmed: 34450180
pii: S0016-5085(21)03413-2
doi: 10.1053/j.gastro.2021.08.033
pmc: PMC8796698
mid: NIHMS1735965
pii:
doi:
Substances chimiques
Biomarkers
0
Types de publication
Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2014-2029.e14Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK116074
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK092421
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007290
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2021 AGA Institute. Published by Elsevier Inc. All rights reserved.
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