An in vivo model of focal light emitting diode-induced cone photoreceptor phototoxicity in adult pigmented mice: Protection with bFGF.
Animals
Arrestins
/ metabolism
Calcium-Binding Proteins
/ metabolism
Disease Models, Animal
Drug Therapy, Combination
Female
Fibroblast Growth Factor 2
/ therapeutic use
Intravitreal Injections
Light
/ adverse effects
Mice
Mice, Inbred C57BL
Microfilament Proteins
/ metabolism
Microscopy, Fluorescence
Minocycline
/ therapeutic use
Radiation Injuries, Experimental
/ diagnostic imaging
Retinal Cone Photoreceptor Cells
/ radiation effects
Retinal Degeneration
/ diagnostic imaging
Retinal Pigment Epithelium
/ metabolism
Tomography, Optical Coherence
Adult pigmented mice
Arrestin
Cone photoreceptor
LED induced Phototoxicity
Microglia activation
Minocycline
Neuroprotection
Retinal pigmented epithelium
bFGF
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
01
06
2021
revised:
18
08
2021
accepted:
20
08
2021
pubmed:
28
8
2021
medline:
6
11
2021
entrez:
27
8
2021
Statut:
ppublish
Résumé
To develop a model of focal injury by blue light-emitting diode (LED)-induced phototoxicity (LIP) in pigmented mouse retinas and to study the effects on cone, Iba-1 In anesthetized dark-adapted adult female pigmented C57BL/6 mice, left pupils were dilated and the eye exposed to LIP (500 lux, 45 s). The retina was monitored longitudinally in vivo with SD-OCT for 7 days (d). Ex vivo, the effects of LIP and its protection with bFGF (0.5 μg) administered alone or combined with minocycline (45 mg/kg) were studied in immunolabeled arrestin-cone outer segments (a LIP caused a focal lesion within the superior-temporal retina with retinal thinning, particularly the outer retinal layers (116.5 ± 2.9 μm to 36.8 ± 6.3 μm at 7d), and with progressive diminution of a LIP resulted in progressive outer retinal damage affecting the OS cone population and RPE. Administration of bFGF increased a
Identifiants
pubmed: 34450185
pii: S0014-4835(21)00312-2
doi: 10.1016/j.exer.2021.108746
pii:
doi:
Substances chimiques
Aif1 protein, mouse
0
Arrestins
0
Calcium-Binding Proteins
0
Microfilament Proteins
0
arrestin 1 protein, mouse
0
Fibroblast Growth Factor 2
103107-01-3
Minocycline
FYY3R43WGO
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108746Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.