Microvesicles transfer mitochondria and increase mitochondrial function in brain endothelial cells.
BBB protection
Exosomes
Extracellular vesicles
Ischemic stroke
Microvesicles
Mitochondrial function
Mitochondrial transfer
Journal
Journal of controlled release : official journal of the Controlled Release Society
ISSN: 1873-4995
Titre abrégé: J Control Release
Pays: Netherlands
ID NLM: 8607908
Informations de publication
Date de publication:
10 10 2021
10 10 2021
Historique:
received:
20
04
2021
revised:
31
07
2021
accepted:
21
08
2021
pubmed:
28
8
2021
medline:
20
11
2021
entrez:
27
8
2021
Statut:
ppublish
Résumé
We have demonstrated, for the first time that microvesicles, a sub-type of extracellular vesicles (EVs) derived from hCMEC/D3: a human brain endothelial cell (BEC) line transfer polarized mitochondria to recipient BECs in culture and to neurons in mice acute brain cortical and hippocampal slices. This mitochondrial transfer increased ATP levels by 100 to 200-fold (relative to untreated cells) in the recipient BECs exposed to oxygen-glucose deprivation, an in vitro model of cerebral ischemia. We have also demonstrated that transfer of microvesicles, the larger EV fraction, but not exosomes resulted in increased mitochondrial function in hypoxic endothelial cultures. Gene ontology and pathway enrichment analysis of EVs revealed a very high association to glycolysis-related processes. In comparison to heterotypic macrophage-derived EVs, BEC-derived EVs demonstrated a greater selectivity to transfer mitochondria and increase endothelial cell survival under ischemic conditions.
Identifiants
pubmed: 34450196
pii: S0168-3659(21)00444-2
doi: 10.1016/j.jconrel.2021.08.038
pmc: PMC8526414
mid: NIHMS1735416
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
505-526Subventions
Organisme : NCI NIH HHS
ID : P30 CA047904
Pays : United States
Organisme : NINDS NIH HHS
ID : R25 NS100118
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007635
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
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