Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila.
Animals
Cell Survival
/ genetics
DNA Topoisomerases, Type II
/ metabolism
Drosophila Proteins
/ metabolism
Drosophila melanogaster
/ cytology
Epistasis, Genetic
Female
Green Fluorescent Proteins
/ metabolism
Intracellular Signaling Peptides and Proteins
/ metabolism
Male
Organogenesis
/ genetics
Phenotype
RNA Interference
Wings, Animal
/ growth & development
Journal
Cell death & disease
ISSN: 2041-4889
Titre abrégé: Cell Death Dis
Pays: England
ID NLM: 101524092
Informations de publication
Date de publication:
27 08 2021
27 08 2021
Historique:
received:
20
01
2021
accepted:
12
08
2021
revised:
28
07
2021
entrez:
28
8
2021
pubmed:
29
8
2021
medline:
29
1
2022
Statut:
epublish
Résumé
Regulation of cell survival is critical for organ development. Translationally controlled tumor protein (TCTP) is a conserved protein family implicated in the control of cell survival during normal development and tumorigenesis. Previously, we have identified a human Topoisomerase II (TOP2) as a TCTP partner, but its role in vivo has been unknown. To determine the significance of this interaction, we examined their roles in developing Drosophila organs. Top2 RNAi in the wing disc leads to tissue reduction and caspase activation, indicating the essential role of Top2 for cell survival. Top2 RNAi in the eye disc also causes loss of eye and head tissues. Tctp RNAi enhances the phenotypes of Top2 RNAi. The depletion of Tctp reduces Top2 levels in the wing disc and vice versa. Wing size is reduced by Top2 overexpression, implying that proper regulation of Top2 level is important for normal organ development. The wing phenotype of Tctp RNAi is partially suppressed by Top2 overexpression. This study suggests that mutual regulation of Tctp and Top2 protein levels is critical for cell survival during organ development.
Identifiants
pubmed: 34453033
doi: 10.1038/s41419-021-04091-y
pii: 10.1038/s41419-021-04091-y
pmc: PMC8397738
doi:
Substances chimiques
Drosophila Proteins
0
Intracellular Signaling Peptides and Proteins
0
TCTP protein, Drosophila
0
Green Fluorescent Proteins
147336-22-9
DNA Topoisomerases, Type II
EC 5.99.1.3
Top2 protein, Drosophila
EC 5.99.1.3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
811Subventions
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2014K1A1A2042982
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2017R1A2B3007516
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2014K1A1A2042982
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2017R1A2B3007516
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2014K1A1A2042982
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2017R1A2B3007516
Informations de copyright
© 2021. The Author(s).
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