Protein adsorption/desorption dynamics on Ca-enriched titanium surfaces: biological implications.
Bioinorganic chemistry
Blood clotting
Dental implants
Osseointegration
Proteomics
Journal
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
ISSN: 1432-1327
Titre abrégé: J Biol Inorg Chem
Pays: Germany
ID NLM: 9616326
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
27
01
2021
accepted:
17
07
2021
pubmed:
29
8
2021
medline:
15
12
2021
entrez:
28
8
2021
Statut:
ppublish
Résumé
Calcium ions are used in the development of biomaterials for the promotion of coagulation, bone regeneration, and implant osseointegration. Upon implantation, the time-dependent release of calcium ions from titanium implant surfaces modifies the physicochemical characteristics at the implant-tissue interface and thus, the biological responses. The aim of this study is to examine how the dynamics of protein adsorption on these surfaces change over time. Titanium discs with and without Ca were incubated with human serum for 2 min, 180 min, and 960 min. The layer of proteins attached to the surface was characterised using nLC-MS/MS. The adsorption kinetics was different between materials, revealing an increased adsorption of proteins associated with coagulation and immune responses prior to Ca release. Implant-blood contact experiments confirmed the strong coagulatory effect for Ca surfaces. We employed primary human alveolar osteoblasts and THP-1 monocytes to study the osteogenic and inflammatory responses. In agreement with the proteomic results, Ca-enriched surfaces showed a significant initial inflammation that disappeared once the calcium was released. The distinct protein adsorption/desorption dynamics found in this work demonstrated to be useful to explain the differential biological responses between the titanium and Ca-ion modified implant surfaces.
Identifiants
pubmed: 34453217
doi: 10.1007/s00775-021-01886-4
pii: 10.1007/s00775-021-01886-4
pmc: PMC8437886
doi:
Substances chimiques
Biocompatible Materials
0
Cytokines
0
Proteins
0
Titanium
D1JT611TNE
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
715-726Informations de copyright
© 2021. The Author(s).
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