Flowcytometric and ImageStream Rna-Fish Gene Expression, Quantification and Phenotypic Characterization of Blood Sporozoites and Sporozoites From Human Malaria Species.
Plasmodium
RNA-FISH
flow analyses
Journal
The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675
Informations de publication
Date de publication:
04 05 2022
04 05 2022
Historique:
received:
12
05
2021
accepted:
27
08
2021
pubmed:
29
8
2021
medline:
10
5
2022
entrez:
28
8
2021
Statut:
ppublish
Résumé
We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.
Identifiants
pubmed: 34453537
pii: 6359056
doi: 10.1093/infdis/jiab431
pmc: PMC9071310
doi:
Substances chimiques
Protozoan Proteins
0
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1621-1625Commentaires et corrections
Type : ErratumIn
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America.
Références
Antimicrob Agents Chemother. 2018 Feb 23;62(3):
pubmed: 29311064
Nat Protoc. 2017 Oct;12(10):2029-2049
pubmed: 28880280
PLoS Biol. 2020 May 4;18(5):e3000711
pubmed: 32365102
Methods Mol Biol. 2013;923:335-51
pubmed: 22990790
Science. 2018 Jan 5;359(6371):48-55
pubmed: 29302006
J Allergy Clin Immunol. 2021 Feb;147(2):740-743.e9
pubmed: 32562707
PLoS One. 2012;7(7):e40798
pubmed: 22844411
J Immunol Methods. 2011 Mar 31;367(1-2):1-16
pubmed: 21296083
Parasitology. 1983 Dec;87 (Pt 3):439-45
pubmed: 6361667
Blood. 2009 Oct 22;114(17):3652-5
pubmed: 19706885
J Parasitol. 2005 Jun;91(3):484-6
pubmed: 16108535
Nat Commun. 2019 Aug 12;10(1):3635
pubmed: 31406175
mBio. 2018 May 8;9(3):
pubmed: 29739900
Blood. 2020 Sep 17;136(12):1381-1393
pubmed: 32589714