Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production.
Calcium alginate bead
Immobilized culture
Porphyridium cruentum
Semi-continuous culture
Sulfated polysaccharides
Journal
Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
14
07
2021
revised:
17
08
2021
accepted:
17
08
2021
pubmed:
29
8
2021
medline:
7
10
2021
entrez:
28
8
2021
Statut:
ppublish
Résumé
In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production. The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively. Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control. Similarly, nutrient deficiencies in combination with immobilization increased daily SPs productivity by about twice that of the control. The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control. This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.
Identifiants
pubmed: 34454230
pii: S0960-8524(21)01157-3
doi: 10.1016/j.biortech.2021.125816
pii:
doi:
Substances chimiques
Polysaccharides
0
Sulfates
0
Sulfur Oxides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
125816Informations de copyright
Copyright © 2021. Published by Elsevier Ltd.