A cloth-based hybridization array system for rapid detection of the food- and waterborne protozoan parasites
Colorimetric detection
Foodborne parasites
Rapid detection
Waterborne parasites
Journal
Food and waterborne parasitology
ISSN: 2405-6766
Titre abrégé: Food Waterborne Parasitol
Pays: Netherlands
ID NLM: 101762332
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
06
05
2021
revised:
03
08
2021
accepted:
06
08
2021
entrez:
30
8
2021
pubmed:
31
8
2021
medline:
31
8
2021
Statut:
epublish
Résumé
Protozoan parasites in food or water samples are generally detected using microscopy or PCR followed by Sanger sequencing. However, microscopy is subjective, requires a high degree of expertise and has limited sensitivity, while DNA sequencing requires expensive and specialized equipment and facilities. This study describes a cloth-based hybridization array system (CHAS) that is an alternative to Sanger sequencing to confirm PCR-positive samples. CHAS is an inexpensive, rapid and reliable method for the simultaneous detection of multiple protozoan parasite species based on the colorimetric detection of PCR amplicons on a polyester cloth. PCR primers and CHAS hybridization probes were developed to detect the protozoan parasites
Identifiants
pubmed: 34458600
doi: 10.1016/j.fawpar.2021.e00130
pii: S2405-6766(21)00021-4
pmc: PMC8379661
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e00130Informations de copyright
Crown Copyright © 2021 Published by Elsevier Inc. on behalf of International Association of Food and Waterborne Parasitology.
Déclaration de conflit d'intérêts
The authors would like to thank Dr. Burton Blais and Martine Gauthier, Canadian Food Inspection Agency, for providing CHAS cloths and expert advice. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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