Modifications of antifungal sensibility testing as suggested by CLSI document M27-A4: proposal for using different culture medium and buffer.
Microbroth dilution
Proposal
Protocol
RPMI-1640
Yeast
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
25
05
2021
revised:
05
07
2021
accepted:
07
07
2021
pubmed:
31
8
2021
medline:
14
1
2022
entrez:
30
8
2021
Statut:
ppublish
Résumé
A common strategy in antifungal susceptibility testing is the utilization of the standardized protocol based on the microbroth dilution assay approach as described by the Clinical Laboratory Standards Institute (CLSI) (M27-A4). One major problem for laboratories in resource-limited countries with this protocol arises from the use of expensive culture media like RPMI-1640 and 3-N-morpholinopropanesulfonic acid (MOPS) buffer. One approach of circumventing this problem in cases of economic need is the evaluation of alternative culture media and buffers. The overall goal of this work was to investigate the influence of modifications in the protocol M27-A4 on diagnostic reliability. We performed univariate analyses evaluating (1) 2 different culture media (YNB and modified SAB); (2) three different buffers (sodium bicarbonate, Tris-HCL, and phosphate), as well as the influence of inoculum concentration (10
Identifiants
pubmed: 34461499
pii: S0732-8893(21)00181-4
doi: 10.1016/j.diagmicrobio.2021.115488
pii:
doi:
Substances chimiques
Antifungal Agents
0
Buffers
0
Culture Media
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115488Informations de copyright
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