MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP.
GPCR
MicroED
ion-beam milling
lipidic cubic phase
membrane proteins
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
07 09 2021
07 09 2021
Historique:
entrez:
31
8
2021
pubmed:
1
9
2021
medline:
8
1
2022
Statut:
ppublish
Résumé
G protein-coupled receptors (GPCRs), or seven-transmembrane receptors, are a superfamily of membrane proteins that are critically important to physiological processes in the human body. Determining high-resolution structures of GPCRs without bound cognate signaling partners, such as a G protein, requires crystallization in lipidic cubic phase (LCP). GPCR crystals grown in LCP are often too small for traditional X-ray crystallography. These microcrystals are ideal for investigation by microcrystal electron diffraction (MicroED), but the gel-like nature of LCP makes traditional approaches to MicroED sample preparation insurmountable. Here, we show that the structure of a human A
Identifiants
pubmed: 34462357
pii: 2106041118
doi: 10.1073/pnas.2106041118
pmc: PMC8433539
pii:
doi:
Substances chimiques
Lipids
0
Receptors, G-Protein-Coupled
0
Receptors, Purinergic P1
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM136508
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM124152
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM127086
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no competing interest.
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