Campylobacter jejuni genotypes are associated with post-infection irritable bowel syndrome in humans.


Journal

Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179

Informations de publication

Date de publication:
30 08 2021
Historique:
received: 18 11 2020
accepted: 13 08 2021
entrez: 31 8 2021
pubmed: 1 9 2021
medline: 15 12 2021
Statut: epublish

Résumé

Campylobacter enterocolitis may lead to post-infection irritable bowel syndrome (PI-IBS) and while some C. jejuni strains are more likely than others to cause human disease, genomic and virulence characteristics promoting PI-IBS development remain uncharacterized. We combined pangenome-wide association studies and phenotypic assays to compare C. jejuni isolates from patients who developed PI-IBS with those who did not. We show that variation in bacterial stress response (Cj0145_phoX), adhesion protein (Cj0628_CapA), and core biosynthetic pathway genes (biotin: Cj0308_bioD; purine: Cj0514_purQ; isoprenoid: Cj0894c_ispH) were associated with PI-IBS development. In vitro assays demonstrated greater adhesion, invasion, IL-8 and TNFα secretion on colonocytes with PI-IBS compared to PI-no-IBS strains. A risk-score for PI-IBS development was generated using 22 genomic markers, four of which were from Cj1631c, a putative heme oxidase gene linked to virulence. Our finding that specific Campylobacter genotypes confer greater in vitro virulence and increased risk of PI-IBS has potential to improve understanding of the complex host-pathogen interactions underlying this condition.

Identifiants

pubmed: 34462533
doi: 10.1038/s42003-021-02554-8
pii: 10.1038/s42003-021-02554-8
pmc: PMC8405632
doi:

Banques de données

figshare
['10.6084/m9.figshare.12493106']

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

1015

Subventions

Organisme : NIDDK NIH HHS
ID : K23 DK103911
Pays : United States
Organisme : NIDDK NIH HHS
ID : R03 DK120745
Pays : United States
Organisme : Medical Research Council
ID : MR/L015080/1
Pays : United Kingdom

Informations de copyright

© 2021. The Author(s).

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Auteurs

Stephanie Peters (S)

Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA.

Ben Pascoe (B)

The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK.

Zuowei Wu (Z)

Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA.

Sion C Bayliss (SC)

The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK.

Ximin Zeng (X)

Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA.

Adam Edwinson (A)

Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA.

Sakteesh Veerabadhran-Gurunathan (S)

Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA.

Selina Jawahir (S)

Minnesota Department of Health, St. Paul, MN, USA.

Jessica K Calland (JK)

The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK.

Evangelos Mourkas (E)

The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK.

Robin Patel (R)

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.

Terra Wiens (T)

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.

Marijke Decuir (M)

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.

David Boxrud (D)

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.

Kirk Smith (K)

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.

Craig T Parker (CT)

United States Department of Agriculture, Albany, CA, USA.

Gianrico Farrugia (G)

Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA.

Qijing Zhang (Q)

Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, USA.

Samuel K Sheppard (SK)

The Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK. s.k.sheppard@bath.ac.uk.

Madhusudan Grover (M)

Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA. Grover.Madhusudan@mayo.edu.

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