Inexpensive workflow for simultaneous monitoring of HIV viral load and detection of SARS-CoV-2 infection.
Journal
medRxiv : the preprint server for health sciences
Titre abrégé: medRxiv
Pays: United States
ID NLM: 101767986
Informations de publication
Date de publication:
24 Aug 2021
24 Aug 2021
Historique:
entrez:
31
8
2021
pubmed:
1
9
2021
medline:
1
9
2021
Statut:
epublish
Résumé
COVID-19 pandemic interrupted routine care for individuals living with HIV, putting them at risk of becoming virologically unsuppressed and ill. Often they are at high risk for exposure to SARS-CoV-2 infection and severe disease once infected. For this population, it is urgent to closely monitor HIV plasma viral load ( We have developed a non-proprietary method to isolate RNA from plasma, nasal secretions ( In-house extraction had a detection limit of 200-copies/mL for HIV and 100-copies/mL for SARS-CoV-2. In-house and commercial methods yielded positively correlated HIV VL (R Our low-cost workflow for molecular testing of HIV and SARS-CoV-2 could serve as an alternative to current standard assays for laboratories in low-resource settings.
Sections du résumé
BACKGROUND
BACKGROUND
COVID-19 pandemic interrupted routine care for individuals living with HIV, putting them at risk of becoming virologically unsuppressed and ill. Often they are at high risk for exposure to SARS-CoV-2 infection and severe disease once infected. For this population, it is urgent to closely monitor HIV plasma viral load (
METHOD
METHODS
We have developed a non-proprietary method to isolate RNA from plasma, nasal secretions (
RESULTS
RESULTS
In-house extraction had a detection limit of 200-copies/mL for HIV and 100-copies/mL for SARS-CoV-2. In-house and commercial methods yielded positively correlated HIV VL (R
INTERPRETATION
CONCLUSIONS
Our low-cost workflow for molecular testing of HIV and SARS-CoV-2 could serve as an alternative to current standard assays for laboratories in low-resource settings.
Identifiants
pubmed: 34462759
doi: 10.1101/2021.08.18.21256786
pmc: PMC8404901
pii:
doi:
Types de publication
Preprint
Langues
eng
Subventions
Organisme : NIAID NIH HHS
ID : P30 AI027757
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI145486
Pays : United States
Organisme : NIAID NIH HHS
ID : R33 AI140460
Pays : United States
Commentaires et corrections
Type : UpdateIn