Non-enzymatic electrochemical cholesterol sensor based on strong host-guest interactions with a polymer of intrinsic microporosity (PIM) with DFT study.
Cholesterol
DFT calculations
Electrochemical sensor
Methylene blue
Polymer of intrinsic microporosity (PIM)
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
04
06
2021
accepted:
10
08
2021
revised:
05
08
2021
pubmed:
1
9
2021
medline:
22
12
2021
entrez:
31
8
2021
Statut:
ppublish
Résumé
Advances in materials science have accelerated the development of diagnostic tools with the last decade witnessing the development of enzyme-free sensors, owing to the improved stability, low cost and simple fabrication of component materials. However, the specificity of non-enzymatic sensors for certain analytes still represents a challenging task, for example the determination of cholesterol level in blood is vital due to its medical relevance. In this work, a reagent displacement assay for cholesterol sensing in serum samples was developed. It is based on coating of a glassy carbon electrode with a polymer of intrinsic microporosity (PIM) that forms a host-guest complex with methylene blue (MB). In the presence of cholesterol, the MB electroactive probe was displaced due to the stronger association of cholesterol guest to the PIM host. The decrease in the oxidative current was proportional to the cholesterol concentration achieving a detection limit of approximately 0.1 nM. Moreover, to further assist the experimental studies, comprehensive theoretical calculations are also performed by using density functional theory (DFT) calculations.
Identifiants
pubmed: 34462789
doi: 10.1007/s00216-021-03616-w
pii: 10.1007/s00216-021-03616-w
doi:
Substances chimiques
Polymers
0
Carbon
7440-44-0
Cholesterol
97C5T2UQ7J
Methylene Blue
T42P99266K
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6523-6533Informations de copyright
© 2021. Springer-Verlag GmbH Germany, part of Springer Nature.
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