Glucose metabolomic profile during embryogenesis in the tick Rhipicephalus microplus.

Embryogenesis Gluconeogenesis Glucose metabolism Metabolomic Tick

Journal

Metabolomics : Official journal of the Metabolomic Society
ISSN: 1573-3890
Titre abrégé: Metabolomics
Pays: United States
ID NLM: 101274889

Informations de publication

Date de publication:
31 08 2021
Historique:
received: 08 10 2020
accepted: 17 08 2021
entrez: 31 8 2021
pubmed: 1 9 2021
medline: 15 1 2022
Statut: epublish

Résumé

Metabolomic approaches can assess the actual state of an organism's energy metabolism during a specific morphological event, providing a more accurate insight into the correlations between physiology and metabolic regulation. The study of the metabolomic profile aim to identify the largest possible number of biomolecules in a certain organism or specific structures. For this purpose, mass spectrometry (MS) and chromatography have been used in the present study. In this context, the aim of the present work is to evaluate the glucose metabolomic profile during embryogenesis in Rhipicephalus microplus tick, investigating the dynamics of nutrient utilization during tick embryo formation, as well as the control of glucose metabolism. We show that glycogen reserves are preferentially mobilized to sustain the energy-intensive process of embryogenesis. Subsequently, the increase in concentration of specific amino acids indicates that protein degradation would provide carbons to fuel gluconeogenesis, supplying the embryo with sufficient glucose and glycogen during development. Altogether, these results demonstrated the presence of a very refined catabolic and anabolic control during embryogenesis in R. microplus tick, suggesting the pronounced gluconeogenesis as a strategy to secure embryo development. Moreover, this research contributes to the understanding of the mechanisms that control glucose metabolism during tick embryogenesis and may aid the identification of putative targets for novel chemical or immunological control methods, which are essential to improve the prevention of tick infestations.

Identifiants

pubmed: 34463832
doi: 10.1007/s11306-021-01830-2
pii: 10.1007/s11306-021-01830-2
doi:

Substances chimiques

Glycogen 9005-79-2
Glucose IY9XDZ35W2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

79

Informations de copyright

© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Jhenifer Nascimento da Silva (JN)

Grupo de Produtos Naturais de Organismos Aquáticos, Instituto de Biodiversidade e Sustentabilidade (NUPEM), Universidade Federal do Rio de Janeiro, Macaé, RJ, Brazil.
Laboratório Integrado de Bioquímica Hatisaburo Masuda and Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Bloco D, Subsolo, Sala 05, Prédio do CCS. Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil.

Daniel Luiz Reis Simas (DLR)

Fábrica de Árvores Soluções Ambientais, Sitio Anjo Gabriel, Bragança Paulista, São Paulo, SP, Brazil.

Angelica Ribeiro Soares (AR)

Grupo de Produtos Naturais de Organismos Aquáticos, Instituto de Biodiversidade e Sustentabilidade (NUPEM), Universidade Federal do Rio de Janeiro, Macaé, RJ, Brazil.

Heitor Monteiro Duarte (HM)

Grupo de Produtos Naturais de Organismos Aquáticos, Instituto de Biodiversidade e Sustentabilidade (NUPEM), Universidade Federal do Rio de Janeiro, Macaé, RJ, Brazil.

Jorge Moraes (J)

Laboratório Integrado de Bioquímica Hatisaburo Masuda and Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Bloco D, Subsolo, Sala 05, Prédio do CCS. Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil.

Christiano Calixto Conceição (CC)

Laboratório Integrado de Bioquímica Hatisaburo Masuda and Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Bloco D, Subsolo, Sala 05, Prédio do CCS. Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil.

Renato Martins da Silva (RM)

Laboratório Integrado de Bioquímica Hatisaburo Masuda and Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Bloco D, Subsolo, Sala 05, Prédio do CCS. Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil.

Itabajara da Silva Vaz (I)

Centro de Biotecnologia and Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.

Carlos Logullo (C)

Laboratório Integrado de Bioquímica Hatisaburo Masuda and Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Bloco D, Subsolo, Sala 05, Prédio do CCS. Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil. carlos.logullo@bioqmed.ufrj.br.
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, RJ, Brazil. carlos.logullo@bioqmed.ufrj.br.

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