Colonization of Mouse Spermatogonial Cells in Modified Soft Agar Culture System Utilizing Nanofibrous Scaffold: A New Approach.
Adult Germline Stem Cells
Agar
Cell Proliferation
Tissue Scaffolds
Journal
Galen medical journal
ISSN: 2322-2379
Titre abrégé: Galen Med J
Pays: Iran
ID NLM: 101625418
Informations de publication
Date de publication:
2019
2019
Historique:
received:
09
08
2018
revised:
07
09
2018
accepted:
16
09
2018
entrez:
1
9
2021
pubmed:
9
5
2019
medline:
9
5
2019
Statut:
epublish
Résumé
Spermatogonial stem cells (SSCs) are considered in fertility management approaches of prepubertal boys facing cancer therapies. However, in vitro propagation has become an important issue due to a small number of SSCs in testicular tissue. The present study aimed to investigate a modified soft agar culture system by using a nanofibrous scaffold as a new approach to mimic in vivo conditions of SSCs development. The SSCs were isolated from neonate mouse testes, cultured on polycaprolactone scaffold, and covered by a layer of soft agar for 2 weeks. Then, the number and diameter of colonies formed in experimental groups were measured and spermatogonial markers (i.e., Our results indicated that the colonization rate of SSCs was significantly higher in the present modified soft agar culture system (P<0.05). Only The culture system by using nanofibrous scaffold and soft agar as a new culture method suggests the potential of this approach in SSCs enrichment and differentiation strategies for male infertility treatments, as well as in vitro spermatogenesis.
Sections du résumé
BACKGROUND
BACKGROUND
Spermatogonial stem cells (SSCs) are considered in fertility management approaches of prepubertal boys facing cancer therapies. However, in vitro propagation has become an important issue due to a small number of SSCs in testicular tissue. The present study aimed to investigate a modified soft agar culture system by using a nanofibrous scaffold as a new approach to mimic in vivo conditions of SSCs development.
MATERIALS AND METHODS
METHODS
The SSCs were isolated from neonate mouse testes, cultured on polycaprolactone scaffold, and covered by a layer of soft agar for 2 weeks. Then, the number and diameter of colonies formed in experimental groups were measured and spermatogonial markers (i.e.,
RESULTS
RESULTS
Our results indicated that the colonization rate of SSCs was significantly higher in the present modified soft agar culture system (P<0.05). Only
CONCLUSION
CONCLUSIONS
The culture system by using nanofibrous scaffold and soft agar as a new culture method suggests the potential of this approach in SSCs enrichment and differentiation strategies for male infertility treatments, as well as in vitro spermatogenesis.
Identifiants
pubmed: 34466493
doi: 10.31661/gmj.v8i0.1319
pmc: PMC8343708
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e1319Informations de copyright
Copyright© 2019, Galen Medical Journal.
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