Effects of Experimental Hyperthyroidism on Collagen IV and Laminin-α5 Gene Expression in Balb/C Mouse Seminiferous Tubules.
Collagen Type IV
Gene Expression
Hyperthyroidism
Laminin
Journal
Galen medical journal
ISSN: 2322-2379
Titre abrégé: Galen Med J
Pays: Iran
ID NLM: 101625418
Informations de publication
Date de publication:
2019
2019
Historique:
received:
27
09
2018
revised:
16
10
2018
accepted:
12
06
2019
entrez:
1
9
2021
pubmed:
9
11
2019
medline:
9
11
2019
Statut:
epublish
Résumé
Hyperthyroidism is one of the disorders of the thyroid gland, an organ that controls the cellular and molecular behaviors of the seminiferous tubule basement membrane (BM), and ultimately, influences its evolutionary process. We aimed to investigate the effects of hyperthyroidism on immunohistochemical characteristics and gene expression levels of collagen IV and laminin-α5 in seminiferous tubules BM of Balb/C mice. Twenty male Balb/C mice were divided into experimental and control groups. The experimental group received 500 mg/l of levothyroxine (L-thyroxine) diluted in drinking water for two months to inducing hyperthyroidism, which was confirmed by radioimmunoassay. At the end of the study, the mice were sacrificed, and their testes were extracted for immunohistochemistry and real-time polymerase chain reaction assays. Although a weak reaction was observed in the experimental specimens, no significant enhancement was noted in color intensity of type IV collagen in the seminiferous tubules BM of the experimental group as compared to the control group (P>0.05). Collagen IV gene expression results in the experimental group were not significantly different from the controls (P>0.05). Thus, there was a significant increase in laminin α5 gene expression compared to the control group (P=0.016). Considering the key role of collagen IV and laminin-α
Sections du résumé
BACKGROUND
BACKGROUND
Hyperthyroidism is one of the disorders of the thyroid gland, an organ that controls the cellular and molecular behaviors of the seminiferous tubule basement membrane (BM), and ultimately, influences its evolutionary process. We aimed to investigate the effects of hyperthyroidism on immunohistochemical characteristics and gene expression levels of collagen IV and laminin-α5 in seminiferous tubules BM of Balb/C mice.
MATERIALS AND METHODS
METHODS
Twenty male Balb/C mice were divided into experimental and control groups. The experimental group received 500 mg/l of levothyroxine (L-thyroxine) diluted in drinking water for two months to inducing hyperthyroidism, which was confirmed by radioimmunoassay. At the end of the study, the mice were sacrificed, and their testes were extracted for immunohistochemistry and real-time polymerase chain reaction assays.
RESULTS
RESULTS
Although a weak reaction was observed in the experimental specimens, no significant enhancement was noted in color intensity of type IV collagen in the seminiferous tubules BM of the experimental group as compared to the control group (P>0.05). Collagen IV gene expression results in the experimental group were not significantly different from the controls (P>0.05). Thus, there was a significant increase in laminin α5 gene expression compared to the control group (P=0.016).
CONCLUSION
CONCLUSIONS
Considering the key role of collagen IV and laminin-α
Identifiants
pubmed: 34466503
doi: 10.31661/gmj.v8i0.1369
pmc: PMC8343772
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e1369Informations de copyright
Copyright© 2019, Galen Medical Journal.
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