Co-localization of nociceptive markers in the lumbar dorsal root ganglion and spinal cord of dromedary camel.
Animals
Calcitonin Gene-Related Peptide
/ metabolism
Camelus
/ metabolism
Ganglia, Spinal
/ metabolism
Immunohistochemistry
Lumbosacral Region
/ innervation
Male
Neurons, Afferent
/ physiology
Nociception
Spinal Cord
/ metabolism
Spinal Cord Dorsal Horn
/ metabolism
TRPV Cation Channels
/ metabolism
AB_2315370
AB_51814
AB_725809
AB_91824
CCK
CGRP
DRG
IB4
NOS
NPY
TRPV1
dromedary camel
spinal cord
Journal
The Journal of comparative neurology
ISSN: 1096-9861
Titre abrégé: J Comp Neurol
Pays: United States
ID NLM: 0406041
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
revised:
19
08
2021
received:
23
06
2021
accepted:
26
08
2021
pubmed:
2
9
2021
medline:
22
3
2022
entrez:
1
9
2021
Statut:
ppublish
Résumé
Nociceptive markers in mice have been identified in two distinct peptidergic and nonpeptidergic neurons in the dorsal root ganglion (DRG) and distributed in different laminae of the dorsal horn of the spinal cord. Recently, however, a study in humans showed a significant overlapping in these two populations. In this study, we investigated the distribution of various nociceptive markers in the lumbar DRG and spinal cord of the dromedary camel. Immunohistochemical data showed a remarkable percentage of total neurons in the DRG expressed IB4 binding (54.5%), calcitonin gene-related peptide (CGRP; 49.5%), transient receptor potential vanilloid 1 (TRPV1; 48.2%), and nitric oxide synthase (NOS; 30.6%). The co-localization data showed that 89.6% and 74.0% of CGRP- and TRPV1-labeled neurons, respectively, were IB4 positive. In addition, 61.6% and 84.2% of TRPV1- and NOS-immunoreactive neurons, respectively, were also co-localized with CGRP. The distribution of IB4, CGRP, TRPV1, substance P, and NOS immunoreactivities in the spinal cord were observed in lamina I and outer lamina II (IIo). Quantitative data showed that 82.4% of IB4-positive nerve terminals in laminae I and IIo were co-localized with CGRP, and 86.0% of CGRP-labeled terminals were co-localized with IB4. Similarly, 85.1% of NOS-labeled nerve terminals were co-localized with CGRP. No neuropeptide Y (NPY) or cholecystokinin (CCK) immunoreactivities were detected in the DRG, and no co-localization between IB4, NPY, and CCK were observed in the spinal cord. Our results demonstrate marked convergence of nociceptive markers in the primary afferent neurons in camels, which is similar to humans rather than the mouse. The data also emphasizes the importance of interspecies differences when selecting ideal animal models for studying nociception and treating chronic pain.
Substances chimiques
TRPV Cation Channels
0
TRPV1 receptor
0
Calcitonin Gene-Related Peptide
JHB2QIZ69Z
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3710-3725Informations de copyright
© 2021 Wiley Periodicals LLC.
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