YB-1 unwinds mRNA secondary structures in vitro and negatively regulates stress granule assembly in HeLa cells.
Adenosine Triphosphate
/ metabolism
Arsenites
/ toxicity
Base Pairing
/ genetics
Cell Line, Tumor
HeLa Cells
Humans
Inverted Repeat Sequences
/ genetics
Peptide Chain Initiation, Translational
/ genetics
RNA, Messenger
/ genetics
Ribosomes
/ metabolism
Stress Granules
/ metabolism
Y-Box-Binding Protein 1
/ metabolism
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
27 09 2021
27 09 2021
Historique:
accepted:
16
08
2021
revised:
12
08
2021
received:
25
06
2021
pubmed:
2
9
2021
medline:
21
12
2021
entrez:
1
9
2021
Statut:
ppublish
Résumé
In the absence of the scanning ribosomes that unwind mRNA coding sequences and 5'UTRs, mRNAs are likely to form secondary structures and intermolecular bridges. Intermolecular base pairing of non polysomal mRNAs is involved in stress granule (SG) assembly when the pool of mRNAs freed from ribosomes increases during cellular stress. Here, we unravel the structural mechanisms by which a major partner of dormant mRNAs, YB-1 (YBX1), unwinds mRNA secondary structures without ATP consumption by using its conserved cold-shock domain to destabilize RNA stem/loops and its unstructured C-terminal domain to secure RNA unwinding. At endogenous levels, YB-1 facilitates SG disassembly during arsenite stress recovery. In addition, overexpression of wild-type YB-1 and to a lesser extent unwinding-defective mutants inhibit SG assembly in HeLa cells. Through its mRNA-unwinding activity, YB-1 may thus inhibit SG assembly in cancer cells and package dormant mRNA in an unfolded state, thus preparing mRNAs for translation initiation.
Identifiants
pubmed: 34469566
pii: 6362110
doi: 10.1093/nar/gkab748
pmc: PMC8464072
doi:
Substances chimiques
Arsenites
0
RNA, Messenger
0
Y-Box-Binding Protein 1
0
YBX1 protein, human
0
Adenosine Triphosphate
8L70Q75FXE
arsenite
N5509X556J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10061-10081Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
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