Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells.


Journal

ACS central science
ISSN: 2374-7943
Titre abrégé: ACS Cent Sci
Pays: United States
ID NLM: 101660035

Informations de publication

Date de publication:
25 Aug 2021
Historique:
received: 02 06 2021
entrez: 2 9 2021
pubmed: 3 9 2021
medline: 3 9 2021
Statut: ppublish

Résumé

New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale

Identifiants

pubmed: 34471685
doi: 10.1021/acscentsci.1c00670
pmc: PMC8393207
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1419-1426

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM134963
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM118486
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK045735
Pays : United States
Organisme : Wellcome Trust
ID : 203285/Z/16/Z
Pays : United Kingdom
Organisme : NIBIB NIH HHS
ID : T32 EB019941
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM131372
Pays : United States

Informations de copyright

© 2021 The Authors. Published by American Chemical Society.

Déclaration de conflit d'intérêts

The authors declare the following competing financial interest(s): J.B. discloses significant financial interest in Bruker Corp. and Hamamatsu Photonics.

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Auteurs

Jonathan Tyson (J)

Department of Chemistry, University of California, Berkeley, California 94720, United States.
Department of Chemistry, Yale University, New Haven, Connecticut 06520, United States.

Kevin Hu (K)

Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, United States.
Department of Biomedical Engineering, Yale University, New Haven, Connecticut 06511, United States.

Shuai Zheng (S)

Department of Chemistry, University of California, Berkeley, California 94720, United States.

Phylicia Kidd (P)

Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, United States.

Neville Dadina (N)

Department of Chemistry, University of California, Berkeley, California 94720, United States.

Ling Chu (L)

Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, United States.
Department of Chemistry, Yale University, New Haven, Connecticut 06520, United States.

Derek Toomre (D)

Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, United States.
Nanobiology Institute, Yale University, West Haven, Connecticut 06516, United States.

Joerg Bewersdorf (J)

Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut 06510, United States.
Department of Biomedical Engineering, Yale University, New Haven, Connecticut 06511, United States.
Kavli Institute for Neuroscience, Yale School of Medicine, New Haven, Connecticut 06510, United States.
Nanobiology Institute, Yale University, West Haven, Connecticut 06516, United States.

Alanna Schepartz (A)

Department of Chemistry, University of California, Berkeley, California 94720, United States.
Department of Molecular and Cellular Biology, University of California, Berkeley, California 94720, United States.
California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, California 94720, United States.
Department of Chemistry, Yale University, New Haven, Connecticut 06520, United States.
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, United States.

Classifications MeSH