UV Resonance Raman explores protein structural modification upon fibrillation and ligand interaction.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
19 10 2021
19 10 2021
Historique:
received:
29
09
2020
revised:
28
06
2021
accepted:
10
08
2021
pubmed:
3
9
2021
medline:
3
11
2021
entrez:
2
9
2021
Statut:
ppublish
Résumé
Amyloids are proteinaceous deposits considered an underlying pathological hallmark of several degenerative diseases. The mechanism of amyloid formation and its inhibition still represent challenging issues, especially when protein structure cannot be investigated by classical biophysical techniques as for the intrinsically disordered proteins (IDPs). In this view, the need to find an alternative way for providing molecular and structural information regarding IDPs prompted us to set a novel, to our knowledge, approach focused on UV Resonance Raman (UVRR) spectroscopy. To test its applicability, we study the fibrillation of hen-egg white lysozyme (HEWL) and insulin as well as their interaction with resveratrol, employing also intrinsic fluorescence spectroscopy, Fourier transform infrared (FTIR) spectroscopy, and atomic force microscopy (AFM). The increasing of the β-sheet structure content at the end of protein fibrillation probed by FTIR occurs simultaneously with a major solvent exposure of tryptophan (Trp) and tyrosine (Tyr) residues of HEWL and insulin, respectively, as revealed by UVRR and intrinsic fluorescence spectroscopy. However, because the latter technique is successfully used when proteins naturally contain Trp residues, it shows poor performances in the case of insulin, and the information regarding its tertiary structure is exclusively provided by UVRR spectroscopy. The presence of an increased concentration of resveratrol induces mild changes in the secondary structure of both protein fibrils while remodeling HEWL fibril length and promoting the formation of amorphous aggregates in the case of insulin. Although the intrinsic fluorescence spectra of proteins are hidden by resveratrol signal, UVRR Trp and Tyr bands are resonantly enhanced, showing a good sensitivity to the presence of resveratrol and marking a modification in the noncovalent interactions in which they are involved. Our findings demonstrate that UVRR is successfully employed in the study of aggregation-prone proteins and of their interaction with ligands, especially in the case of Trp-lacking proteins.
Identifiants
pubmed: 34474016
pii: S0006-3495(21)00698-6
doi: 10.1016/j.bpj.2021.08.032
pmc: PMC8553600
pii:
doi:
Substances chimiques
Amyloid
0
Intrinsically Disordered Proteins
0
Ligands
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4575-4589Informations de copyright
Copyright © 2021 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Références
Prog Biophys Mol Biol. 1993;59(1):23-56
pubmed: 8419985
J Neurochem. 2006 Apr;97(1):105-15
pubmed: 16524383
Chem Rev. 2012 May 9;112(5):2604-28
pubmed: 22335827
Biochemistry. 2006 Feb 28;45(8):2752-60
pubmed: 16489768
J Phys Chem B. 2015 Dec 17;119(50):15395-406
pubmed: 26569375
Biochemistry. 1988 Jan 12;27(1):88-94
pubmed: 3349046
Biomolecules. 2015 Apr 17;5(2):505-27
pubmed: 25898401
Elife. 2018 Jul 03;7:
pubmed: 29969391
J Pharm Sci. 2001 Jan;90(1):29-37
pubmed: 11064376
Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):1937-42
pubmed: 22308365
PLoS One. 2012;7(6):e36989
pubmed: 22675475
J Phys Chem Lett. 2014 Jun 5;5(11):1984-1993
pubmed: 24932380
Biochemistry. 2006 Jan 10;45(1):34-41
pubmed: 16388578
Trends Analyt Chem. 2018 Jun;103:223-229
pubmed: 32029956
Biopolymers. 2005 Sep;79(1):58-61
pubmed: 15962278
J Mol Biol. 2002 Apr 26;318(2):479-90
pubmed: 12051853
ACS Omega. 2019 Jan 11;4(1):1015-1026
pubmed: 31459377
Biochemistry. 1999 Jun 29;38(26):8196-203
pubmed: 10387065
Biochemistry. 1998 Mar 3;37(9):2854-64
pubmed: 9485436
Biochim Biophys Acta. 2003 Sep 23;1651(1-2):139-45
pubmed: 14499598
J Phys Chem B. 2015 Jan 29;119(4):1265-74
pubmed: 25545790
Food Funct. 2013 Oct;4(10):1474-80
pubmed: 23995676
J Agric Food Chem. 2009 Dec 9;57(23):11391-6
pubmed: 19904937
Biopolymers. 2003;72(5):305-17
pubmed: 12949821
J Am Chem Soc. 2007 Sep 12;129(36):11002-3
pubmed: 17705492
Chem Commun (Camb). 2010 Jun 28;46(24):4249-51
pubmed: 20532285
Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):18990-5
pubmed: 19864624
Angew Chem Int Ed Engl. 2005 Jul 18;44(29):4631-5
pubmed: 15977271
Methods. 2010 Sep;52(1):23-37
pubmed: 20580825
Biochemistry. 2003 Dec 9;42(48):14234-41
pubmed: 14640691
Biophys Chem. 2011 Oct;158(2-3):96-103
pubmed: 21652140
Protein Sci. 2000 Sep;9(9):1700-8
pubmed: 11045616
Biochem Biophys Res Commun. 2011 Dec 2;415(4):675-9
pubmed: 22079288
Biochemistry. 2010 May 11;49(18):3935-46
pubmed: 20369844
Q Rev Biophys. 2002 Nov;35(4):369-430
pubmed: 12621861
EMBO J. 2002 Nov 1;21(21):5682-90
pubmed: 12411486
Biochemistry. 2015 Aug 11;54(31):4770-83
pubmed: 26219819
J Biophotonics. 2008 Aug;1(3):215-29
pubmed: 19412971
Protein Sci. 2000 Oct;9(10):1960-7
pubmed: 11106169
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3590-4
pubmed: 10097081
Nat Chem Biol. 2011 Aug 28;7(10):730-9
pubmed: 21873994
Arch Pharm Res. 2009 Jun;32(6):869-81
pubmed: 19557365
Biochemistry. 1998 Mar 3;37(9):2865-72
pubmed: 9485437
J Am Chem Soc. 2004 Mar 3;126(8):2399-408
pubmed: 14982446
Biophys J. 2001 May;80(5):2093-109
pubmed: 11325713
Nat Struct Mol Biol. 2008 Jun;15(6):558-66
pubmed: 18511942
J Phys Chem B. 2013 Apr 18;117(15):4003-13
pubmed: 23537140
Biochemistry. 1988 Jul 26;27(15):5426-33
pubmed: 3179264
Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):522-46
pubmed: 9761848
J Mol Biol. 2000 Oct 20;303(2):267-78
pubmed: 11023791
Protein Sci. 2004 Dec;13(12):3314-21
pubmed: 15537750
J Mol Biol. 1999 Oct 22;293(2):321-31
pubmed: 10550212
Biochim Biophys Acta. 2005 Feb 25;1707(1):51-66
pubmed: 15721606
Biophys J. 2017 Oct 17;113(8):1685-1696
pubmed: 29045863
Chem Biol Drug Des. 2006 Jan;67(1):27-37
pubmed: 16492146
Biomacromolecules. 2012 May 14;13(5):1503-9
pubmed: 22515261
J Pharm Bioallied Sci. 2013 Apr;5(2):148-53
pubmed: 23833521
Biopolymers. 1975 Apr;14(4):679-94
pubmed: 1156632
Nat Struct Mol Biol. 2016 May;23(5):409-15
pubmed: 27018801
PLoS One. 2014 Apr 16;9(4):e94766
pubmed: 24740323
Biophys J. 2009 Nov 4;97(9):2521-31
pubmed: 19883595
Islets. 2009 Nov-Dec;1(3):210-5
pubmed: 21099274
Biochemistry. 1996 Dec 10;35(49):15822-30
pubmed: 8961946
Biophys J. 2009 Apr 22;96(8):3331-40
pubmed: 19383476
Nat Commun. 2018 Sep 6;9(1):3609
pubmed: 30190461
Biochim Biophys Acta. 2007 Sep;1767(9):1073-101
pubmed: 17692815
Pharm Res. 2006 Jun;23(6):1350-63
pubmed: 16715365
Nat Protoc. 2015 Mar;10(3):382-96
pubmed: 25654756
Proteins. 2013 Jun;81(6):994-1004
pubmed: 23280648
J Am Chem Soc. 2014 Jun 25;136(25):8947-56
pubmed: 24884889
Nat Rev Mol Cell Biol. 2018 Dec;19(12):755-773
pubmed: 30237470
Biochemistry. 2007 Feb 20;46(7):1888-99
pubmed: 17253770
Biophys J. 2005 Jan;88(1):515-26
pubmed: 15489299
J Phys Chem B. 2017 Sep 28;121(38):8935-8945
pubmed: 28851219
Biochemistry. 1991 Jun 18;30(24):5944-54
pubmed: 2043634
J Am Chem Soc. 2007 Jun 6;129(22):6972-3
pubmed: 17500518
J Phys Chem B. 2009 Nov 5;113(44):14769-78
pubmed: 19817473
J Phys Chem B. 2019 Jul 11;123(27):5678-5689
pubmed: 31246474
Biochemistry. 2010 Dec 21;49(50):10623-35
pubmed: 21077643
Int J Biol Macromol. 2016 Nov;92:1049-1056
pubmed: 27506123
Sci Adv. 2019 Jun 28;5(6):eaax2348
pubmed: 31259246
Analyst. 2015 Aug 7;140(15):4967-80
pubmed: 26042229
Pigment Cell Melanoma Res. 2013 May;26(3):300-15
pubmed: 23350640
Int J Biol Macromol. 2018 Aug;115:1157-1164
pubmed: 29727655
Annu Rev Biochem. 2006;75:333-66
pubmed: 16756495
J Mol Biol. 2004 Jul 23;340(5):1153-65
pubmed: 15236974
Analyst. 2009 Jan;134(1):138-47
pubmed: 19082186
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15463-8
pubmed: 14665689
Appl Spectrosc. 2015 Jan;69(1):75-83
pubmed: 25506729
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15871-6
pubmed: 16247008
Neurotoxicology. 2009 Nov;30(6):986-95
pubmed: 19744518
Int J Biol Sci. 2013;9(2):219-27
pubmed: 23459392
Int J Biol Macromol. 2020 Mar 15;147:98-108
pubmed: 31923504
J Mol Biol. 2010 Oct 8;402(5):783-96
pubmed: 20624399
Sci Rep. 2014 Jul 10;4:5648
pubmed: 25008537
Biopolymers. 1986 Mar;25(3):469-87
pubmed: 3697478
PLoS One. 2015 Nov 16;10(11):e0142095
pubmed: 26571264
Biochemistry. 1994 Sep 6;33(35):10725-30
pubmed: 8075073
Nat Commun. 2019 Apr 23;10(1):1864
pubmed: 31015551
J Am Chem Soc. 2012 Aug 15;134(32):13323-9
pubmed: 22813355
Science. 2017 Dec 15;358(6369):1440-1443
pubmed: 29242346