In-vitro thromboelastographic characterization of reconstituted whole blood utilizing cryopreserved platelets.
Journal
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis
ISSN: 1473-5733
Titre abrégé: Blood Coagul Fibrinolysis
Pays: England
ID NLM: 9102551
Informations de publication
Date de publication:
01 Dec 2021
01 Dec 2021
Historique:
pubmed:
4
9
2021
medline:
14
1
2022
entrez:
3
9
2021
Statut:
ppublish
Résumé
Conducting in-vitro thrombosis research presents numerous challenges, the primary of which is working with blood products, whether whole blood or fractionated whole blood, that have limited functional shelf-lives. As a result, being able to significantly prolong the clotting functionality of whole blood via fractionation and recombination promises greater accessibility via resource minimization in the realm of thrombosis research. Whole blood with CPDA1 from healthy volunteers was fractionated and stored as frozen platelet-free plasma (PFP, -20°C), refrigerated packed red blood cells (pRBCs, 4°C) and cryopreserved platelets (-80°C). Subsequent recombination of the above components into their native ratios were tested via thromboelastography (TEG) to capture clotting dynamics over a storage period of 13 weeks in comparison to refrigerated unfractionated WB+CPDA1. Reconstituted whole blood utilizing PFP, pRCBs and cryopreserved platelets were able to maintain clot strength (maximum amplitude) akin to day-0 whole blood even after 13 weeks of storage. Clots formed by reconstituted whole blood exhibited quicker clotting dynamics with nearly two-fold shorter R-times and nearly 1.3-fold increase in fibrin deposition rate as measured by TEG. Storage of fractionated whole blood components, in their respective ideal conditions, provides a means of prolonging the usable life of whole blood for in-vitro thrombosis research. Cryopreserved platelets, when recombined with frozen PFP and refrigerated pRBCs, are able to form clots that nearly mirror the overall clotting profile expected of freshly drawn WB.
Identifiants
pubmed: 34475333
doi: 10.1097/MBC.0000000000001075
pii: 00001721-202112000-00005
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
556-563Informations de copyright
Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.
Références
Devine DV, Serrano K. The platelet storage lesion. Clin Lab Med 2010; 30:475–487.
Hegde S, Akbar H, Zheng Y, Cancelas JA. Towards increasing shelf life and haemostatic potency of stored platelet concentrates. Curr Opin Hematol 2018; 25:500–508.
Levi MJ, Dorffle-Melly GJ, Johnson L, Drouet L, Badimon C. Subcommittee on Animal. Usefulness and limitations of animal models of venous thrombosis. Thromb Haemost 2001; 86:1331–1333.
Cito S, Mazzeo MD, Badimon L. A review of macroscopic thrombus modeling methods. Thromb Res 2013; 131:116–124.
Hunter AJ, Green AR, Cross AJ. Animal models of acute ischaemic stroke: can they predict clinically successful neuroprotective drugs? Trends Pharmacol Sci 1995; 16:123–128.
American Association of Blood Banks. Technical manual of the American Association of Blood Banks. 17th ed.2011; Arlington, VA: Amer Assn of Blood Banks, 1038 p.
Slichter SJ, Jones M, Ransom J, Gettinger I, Jones MK, Christoffel T, et al. Review of in vivo studies of dimethyl sulfoxide cryopreserved platelets. Transfus Med Rev 2014; 28:212–225.
Valeri CR, Feingold H, Marchionni LD. A simple method for freezing human platelets using 6 per cent dimethylsulfoxide and storage at -80 degrees C. Blood 1974; 43:131–136.
Barnard MR, MacGregor H, Ragno G, Pivacek LE, Khuri SF, Michelson AD, et al. Fresh, liquid-preserved, and cryopreserved platelets: adhesive surface receptors and membrane procoagulant activity. Transfusion 1999; 39:880–888.
Marks DC, Johnson L. Assays for phenotypic and functional characterization of cryopreserved platelets. Platelets 2019; 30:48–55.
Tegegn TZ, De Paoli SH, Orecna M, Elhelu OK, Woodle SA, Tarandovskiy ID, et al. Characterization of procoagulant extracellular vesicles and platelet membrane disintegration in DMSO-cryopreserved platelets. J Extracell Vesicles 2016; 5:30422.
Slichter SJ, Dumont LJ, Cancelas JA, Jones M, Gernsheimer TB, Szczepiorkowski ZM, et al. Safety and efficacy of cryopreserved platelets in bleeding patients with thrombocytopenia. Transfusion 2018; 58:2129–2138.
Holley A, Marks DC, Johnson L, Reade MC, Badloe JF, Noorman F. Frozen blood products: clinically effective and potentially ideal for remote Australia. Anaesth Intensive Care 2013; 41:10–19.
Six KR, Compernolle V, Feys HB. Platelet biochemistry and morphology after cryopreservation. Int J Mol Sci 2020; 21:E935.
Six KR, Delabie W, Devreese KMJ, Johnson L, Marks DC, Dumont LJ, et al. Comparison between manufacturing sites shows differential adhesion, activation, and GPIbalpha expression of cryopreserved platelets. Transfusion 2018; 58:2645–2656.
Johnson L, Tan S, Wood B, Davis A, Marks DC. Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions. Transfusion 2016; 56:1807–1818.
Meinke S, Wikman A, Gryfelt G, Hultenby K, Uhlin M, Hoglund P, et al. Cryopreservation of buffy coat-derived platelet concentrates photochemically treated with amotosalen and UVA light. Transfusion 2018; 58:2657–2668.
Johnson L, Coorey CP, Marks DC. The hemostatic activity of cryopreserved platelets is mediated by phosphatidylserine-expressing platelets and platelet microparticles. Transfusion 2014; 54:1917–1926.
Perez-Ferrer A, Navarro-Suay R, Viejo-Llorente A, Alcaide-Martin MJ, de Vicente-Sanchez J, Butta N, et al. In vitro thromboelastometric evaluation of the efficacy of frozen platelet transfusion. Thromb Res 2015; 136:348–353.
Cid J, Escolar G, Galan A, Lopez-Vilchez I, Molina P, Diaz-Ricart M, et al. In vitro evaluation of the hemostatic effectiveness of cryopreserved platelets. Transfusion 2016; 56:580–586.
Othman M, Kaur H. Thromboelastography (TEG). Methods Mol Biol 2017; 1646:533–543.
Amgalan A, Allen T, Othman M, Ahmadzia HK. Systematic review of viscoelastic testing (TEG/ROTEM) in obstetrics and recommendations from the women's SSC of the ISTH. J Thromb Haemost 2020; 18:1813–1838.
Schmidt AE, Israel AK, Refaai MA. The utility of thromboelastography to guide blood product transfusion. Am J Clin Pathol 2019; 152:407–422.
Pretorius E, Swanepoel AC, DeVilliers S, Bester J. Blood clot parameters: thromboelastography and scanning electron microscopy in research and clinical practice. Thromb Res 2017; 154:59–63.
Billett HH. Walker HK, Hall WD, Hurst JW. Hemoglobin and hematocrit. Clinical Methods: The History, Physical, and Laboratory Examinations [Internet] 3rd ed.Boston: Butterworths; 1990; Chapter 151.
Stiff PJ. Walker HK, Hall WD, Hurst JW. Platelets. Clinical Methods: The History, Physical, and Laboratory Examinations [Internet] 3rd ed.Boston: Butterworths; 1990; Chapter 154.
Wang S, Zhao G, Li N, Hou T, Shao X, Tang W, et al. An in vitro study of coagulation properties in refrigerated whole blood and reconstituted whole blood. Vox Sang 2019; 114:694–700.
Shaydakov ME, Sigmon DF, Blebea J. Thromboelastography. In: StatPearls [Internet]. 2021; Treasure Island (FL): StatPearls Publishing, Jan–.
Bose E, Hravnak M. Thromboelastography: a practice summary for nurse practitioners treating hemorrhage. J Nurse Pract 2015; 11:702–709.
Solomon C, Ranucci M, Hochleitner G, Schochl H, Schlimp CJ. Assessing the methodology for calculating platelet contribution to clot strength (platelet component) in thromboelastometry and thrombelastography. Anesth Analg 2015; 121:868–878.
Raj TD. Thromboelastogram I. In: Raj TD, editor. Data interpretation in anesthesia: a clinical guide. Cham: Springer International Publishing; 2017. pp. 161–166.
Bolliger D, Seeberger MD, Tanaka KA. Principles and practice of thromboelastography in clinical coagulation management and transfusion practice. Transfus Med Rev 2012; 26:1–13.
Bowbrick VA, Mikhailidis DP, Stansby G. Value of thromboelastography in the assessment of platelet function. Clin Appl Thromb Hemost 2003; 9:137–142.
Bowbrick VA, Mikhailidis DP, Stansby G. The use of citrated whole blood in thromboelastography. Anesth Analg 2000; 90:1086–1088.
Dias JD, Haney EI, Mathew BA, Lopez-Espina CG, Orr AW, Popovsky MA. New-generation thromboelastography: comprehensive evaluation of citrated and heparinized blood sample storage effect on clot-forming variables. Arch Pathol Lab Med 2017; 141:569–577.
Zeng Z, Fagnon M, Nallan Chakravarthula T, Alves NJ. Fibrin clot formation under diverse clotting conditions: comparing turbidimetry and thromboelastography. Thromb Res 2020; 187:48–55.
Zeng Z, Nallan Chakravarthula T, Alves NJ. Leveraging turbidity and thromboelastography for complementary clot characterization. J Vis Exp 2020; (160):e61519.
Hess JR. Conventional blood banking and blood component storage regulation: opportunities for improvement. Blood Transfus 2010; 8: (Suppl 3): s9–15.
Bontekoe IJ, van der Meer PF, de Korte D. Determination of thromboelastographic responsiveness in stored single-donor platelet concentrates. Transfusion 2014; 54:1610–1618.
van Hout FMA, Bontekoe IJ, de Laleijne LAE, Kerkhoffs JL, de Korte D, Eikenboom J, et al. Comparison of haemostatic function of PAS-C-platelets vs. plasma-platelets in reconstituted whole blood using impedance aggregometry and thromboelastography. Vox Sang 2017; 112:549–556.
Roeloffzen WW, Kluin-Nelemans HC, Bosman L, de Wolf JT. Effects of red blood cells on hemostasis. Transfusion 2010; 50:1536–1544.
Lindahl TL, Ramstrom S. Methods for evaluation of platelet function. Transfus Apher Sci 2009; 41:121–125.
Johnson L, Raynel S, Seghatchian J, Marks DC. Platelet microparticles in cryopreserved platelets: Potential mediators of haemostasis. Transfus Apher Sci 2015; 53:146–152.
Eker I, Yilmaz S, Cetinkaya RA, Pekel A, Unlu A, Gursel O, et al. Generation of platelet microparticles after cryopreservation of apheresis platelet concentrates contributes to hemostatic activity. Turk J Haematol 2017; 34:64–71.
Wood B, Padula MP, Marks DC, Johnson L. Refrigerated storage of platelets initiates changes in platelet surface marker expression and localization of intracellular proteins. Transfusion 2016; 56:2548–2559.
Raynel S, Padula MP, Marks DC, Johnson L. Cryopreservation alters the membrane and cytoskeletal protein profile of platelet microparticles. Transfusion 2015; 55:2422–2432.
Weisel JW, Litvinov RI. Red blood cells: the forgotten player in hemostasis and thrombosis. J Thromb Haemost 2019; 17:271–282.
Perez AG, Rodrigues AA, Luzo AC, Lana JF, Belangero WD, Santana MH. Fibrin network architectures in pure platelet-rich plasma as characterized by fiber radius and correlated with clotting time. J Mater Sci Mater Med 2014; 25:1967–1977.
Collet JP, Lesty C, Montalescot G, Weisel JW. Dynamic changes of fibrin architecture during fibrin formation and intrinsic fibrinolysis of fibrin-rich clots. J Biol Chem 2003; 278:21331–21335.
Blomback B, Carlsson K, Fatah K, Hessel B, Procyk R. Fibrin in human plasma: gel architectures governed by rate and nature of fibrinogen activation. Thromb Res 1994; 75:521–538.
Waldron JM, Duncan GG. Variability of the rate of coagulation of whole blood. Am J Med 1954; 17:365–373.