Integrin α6 β4 on Circulating Tumor Cells of Metastatic Breast Cancer Patients.
Circulating tumor cells
epithelial-to-mesenchymal transition
metastatic breast cancer
α6 β4 integrin
Journal
Advanced biomedical research
ISSN: 2277-9175
Titre abrégé: Adv Biomed Res
Pays: India
ID NLM: 101586897
Informations de publication
Date de publication:
2021
2021
Historique:
received:
03
04
2021
revised:
26
05
2021
accepted:
02
06
2021
entrez:
3
9
2021
pubmed:
4
9
2021
medline:
4
9
2021
Statut:
epublish
Résumé
The detection of circulating tumor cells (CTCs) is prognostic during the disease in women with metastatic breast cancer. Integrins are key role players in nearly every step of cancer progression. In this study, we aimed to analyze integrin alpha6beta4 expression on CTCs isolated from blood samples of patients with advanced breast cancer. In this single-center study, peripheral blood samples from 23 breast cancer patients were obtained and analyzed for the presence of CTCs by EasySep™ Direct Human CTC Enrichment Kit combined with subsequent immunocytochemical staining of anti-cytokeratin and anti-epithelial cell adhesion molecules, and β4 integrin on CTCs. Data were correlated with clinicopathological parameters. CTCs were detected in 100% of the patients. The ratio of integrin β4+ CTC was 61% ± 8% of total CTCs. No significant correlation between histopathological parameters and CTC detection was found. Our results demonstrated the importance of α6 β4 integrin expression on CTCs in distant metastasis.
Sections du résumé
BACKGROUND
BACKGROUND
The detection of circulating tumor cells (CTCs) is prognostic during the disease in women with metastatic breast cancer. Integrins are key role players in nearly every step of cancer progression. In this study, we aimed to analyze integrin alpha6beta4 expression on CTCs isolated from blood samples of patients with advanced breast cancer.
MATERIALS AND METHODS
METHODS
In this single-center study, peripheral blood samples from 23 breast cancer patients were obtained and analyzed for the presence of CTCs by EasySep™ Direct Human CTC Enrichment Kit combined with subsequent immunocytochemical staining of anti-cytokeratin and anti-epithelial cell adhesion molecules, and β4 integrin on CTCs. Data were correlated with clinicopathological parameters.
RESULTS
RESULTS
CTCs were detected in 100% of the patients. The ratio of integrin β4+ CTC was 61% ± 8% of total CTCs. No significant correlation between histopathological parameters and CTC detection was found.
CONCLUSION
CONCLUSIONS
Our results demonstrated the importance of α6 β4 integrin expression on CTCs in distant metastasis.
Identifiants
pubmed: 34476224
doi: 10.4103/abr.abr_76_21
pii: ABR-10-16
pmc: PMC8378442
doi:
Types de publication
Journal Article
Langues
eng
Pagination
16Informations de copyright
Copyright: © 2021 Advanced Biomedical Research.
Déclaration de conflit d'intérêts
There are no conflicts of interest.
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