Sustainable utilization of Gyrinops walla Gaetner: in vitro production of sesquiterpenes by chemical and biological elicitation.
Artificial elicitation
Gyrinops walla
In vitro production
Sesquiterpenes
Sustainability
Journal
Journal, genetic engineering & biotechnology
ISSN: 2090-5920
Titre abrégé: J Genet Eng Biotechnol
Pays: Netherlands
ID NLM: 101317150
Informations de publication
Date de publication:
03 Sep 2021
03 Sep 2021
Historique:
received:
18
01
2021
accepted:
31
05
2021
entrez:
3
9
2021
pubmed:
4
9
2021
medline:
4
9
2021
Statut:
epublish
Résumé
The recent recovery of Gyrinops walla as a potential producer of market-quality agarwood in mature damaged woods and branches has led to the intense illicit felling and exportation of G. walla leading to the verge of extinction from Sri Lankan flora. The sustainable utilization of G. walla undoubtedly enhances the foreign exchange of the country and the non-destructive utilization through tissue culture-based techniques is the only option available for sustainable exploitation and conservation of the vulnerable species. Healthy calli and cell suspensions were chemically and biologically elicited with salicylic acid (SA) and methyl jasmonate (MJ), and the sterilized fungal homogenate (carbohydrate equivalents) of Fusariym oxysporum, Phaeocremonium parasitica, Aspergillus niger, Trichoderma viride, Penicillium commune and Lasidiplodia theobromae fungal strains, respectively. The elicited calli and cell suspensions were harvested at different time periods to extract sesquiterpenes. Sesquiterpenes were produced in calli under chemical elicitors with media concentrations of 10 μM SA, 100 μM SA, 10 mM MJ and 1 mM MJ and cell suspensions under 0.5 μM SA and 0.1 mM MJ. Phaeocremonium parasitica, Trichoderma viride and Lasidiplodia theobromae were more effective in the production of sesquiterpenes in G. walla callus and cell suspension by biological elicitation. The findings of the study led to the conclusion of the possibility of induction of production of sesquiterpenes through elicitation of G. walla calli and cell suspension in an in vitro system for sustainable utilization and conservation endeavours.
Sections du résumé
BACKGROUND
BACKGROUND
The recent recovery of Gyrinops walla as a potential producer of market-quality agarwood in mature damaged woods and branches has led to the intense illicit felling and exportation of G. walla leading to the verge of extinction from Sri Lankan flora. The sustainable utilization of G. walla undoubtedly enhances the foreign exchange of the country and the non-destructive utilization through tissue culture-based techniques is the only option available for sustainable exploitation and conservation of the vulnerable species. Healthy calli and cell suspensions were chemically and biologically elicited with salicylic acid (SA) and methyl jasmonate (MJ), and the sterilized fungal homogenate (carbohydrate equivalents) of Fusariym oxysporum, Phaeocremonium parasitica, Aspergillus niger, Trichoderma viride, Penicillium commune and Lasidiplodia theobromae fungal strains, respectively. The elicited calli and cell suspensions were harvested at different time periods to extract sesquiterpenes.
RESULTS
RESULTS
Sesquiterpenes were produced in calli under chemical elicitors with media concentrations of 10 μM SA, 100 μM SA, 10 mM MJ and 1 mM MJ and cell suspensions under 0.5 μM SA and 0.1 mM MJ. Phaeocremonium parasitica, Trichoderma viride and Lasidiplodia theobromae were more effective in the production of sesquiterpenes in G. walla callus and cell suspension by biological elicitation.
CONCLUSION
CONCLUSIONS
The findings of the study led to the conclusion of the possibility of induction of production of sesquiterpenes through elicitation of G. walla calli and cell suspension in an in vitro system for sustainable utilization and conservation endeavours.
Identifiants
pubmed: 34477991
doi: 10.1186/s43141-021-00187-2
pii: 10.1186/s43141-021-00187-2
pmc: PMC8417190
doi:
Types de publication
Journal Article
Langues
eng
Pagination
134Subventions
Organisme : University Grants Commission - Sri Lanka
ID : OU201601
Informations de copyright
© 2021. The Author(s).
Références
Curr Opin Plant Biol. 2003 Aug;6(4):365-71
pubmed: 12873532
Appl Microbiol Biotechnol. 2001 May;55(4):404-10
pubmed: 11398918
Curr Opin Plant Biol. 1998 Oct;1(5):404-11
pubmed: 10066616
Iran J Pharm Res. 2012 Fall;11(4):1103-9
pubmed: 24250543
Nat Protoc. 2011 Jun;6(6):715-42
pubmed: 21637194
J Nat Med. 2008 Jan;62(1):41-6
pubmed: 18404340
Curr Opin Plant Biol. 2000 Aug;3(4):309-14
pubmed: 10873851
Methods Mol Biol. 1990;6:29-37
pubmed: 21390590
Phytochemistry. 2003 Mar;62(6):901-9
pubmed: 12590117
Plant Physiol. 2010 Dec;154(4):1998-2007
pubmed: 20959422