Impact of temperature on the affinity of SARS-CoV-2 Spike glycoprotein for host ACE2.
ACE2
COVID-19
N501Y
RBD
SARS-CoV-2
Spike glycoproteins
coronavirus
neutralization
temperature
variants of concern
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
09
07
2021
revised:
25
08
2021
accepted:
30
08
2021
pubmed:
4
9
2021
medline:
11
11
2021
entrez:
3
9
2021
Statut:
ppublish
Résumé
The seasonal nature of outbreaks of respiratory viral infections with increased transmission during low temperatures has been well established. Accordingly, temperature has been suggested to play a role on the viability and transmissibility of SARS-CoV-2, the virus responsible for the COVID-19 pandemic. The receptor-binding domain (RBD) of the Spike glycoprotein is known to bind to its host receptor angiotensin-converting enzyme 2 (ACE2) to initiate viral fusion. Using biochemical, biophysical, and functional assays to dissect the effect of temperature on the receptor-Spike interaction, we observed a significant and stepwise increase in RBD-ACE2 affinity at low temperatures, resulting in slower dissociation kinetics. This translated into enhanced interaction of the full Spike glycoprotein with the ACE2 receptor and higher viral attachment at low temperatures. Interestingly, the RBD N501Y mutation, present in emerging variants of concern (VOCs) that are fueling the pandemic worldwide (including the B.1.1.7 (α) lineage), bypassed this requirement. This data suggests that the acquisition of N501Y reflects an adaptation to warmer climates, a hypothesis that remains to be tested.
Identifiants
pubmed: 34478710
pii: S0021-9258(21)00952-2
doi: 10.1016/j.jbc.2021.101151
pmc: PMC8406544
pii:
doi:
Substances chimiques
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Angiotensin-Converting Enzyme 2
EC 3.4.17.23
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
101151Commentaires et corrections
Type : UpdateOf
Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.
Références
Cell. 2021 Jun 24;184(13):3426-3437.e8
pubmed: 33991487
Int J Environ Res Public Health. 2021 Apr 12;18(8):
pubmed: 33921381
Cell. 2021 Apr 29;184(9):2384-2393.e12
pubmed: 33794143
PLoS Pathog. 2021 Apr 22;17(4):e1009500
pubmed: 33886690
PLoS Med. 2006 Jul;3(7):e237
pubmed: 16796401
EMBO J. 1997 Feb 17;16(4):695-705
pubmed: 9049299
Lancet Infect Dis. 2004 Nov;4(11):704-8
pubmed: 15522683
Nat Commun. 2020 Mar 27;11(1):1620
pubmed: 32221306
JAMA Netw Open. 2020 Jun 1;3(6):e2011834
pubmed: 32525550
Viruses. 2020 Sep 29;12(10):
pubmed: 33003587
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
Elife. 2020 Oct 28;9:
pubmed: 33112236
Nat Struct Mol Biol. 2021 Feb;28(2):128-131
pubmed: 33402708
Nat Commun. 2020 Nov 26;11(1):6013
pubmed: 33243994
Can Commun Dis Rep. 2021 Mar 31;47(3):132-138
pubmed: 34012336
J Virol. 1991 Sep;65(9):5007-12
pubmed: 1870209
Nature. 2021 Apr;592(7854):438-443
pubmed: 33690265
Nat Med. 2020 Apr;26(4):450-452
pubmed: 32284615
J Appl Physiol (1985). 1985 Feb;58(2):564-70
pubmed: 3980358
Environ Res. 2021 Apr;195:110874
pubmed: 33610582
Cell. 2020 Apr 16;181(2):281-292.e6
pubmed: 32155444
Sci Immunol. 2020 May 13;5(47):
pubmed: 32404436
Viruses. 2020 Oct 26;12(11):
pubmed: 33114742
Cell. 2020 Aug 20;182(4):812-827.e19
pubmed: 32697968
Comput Struct Biotechnol J. 2021;19:161-167
pubmed: 33343834
Respir Physiol Neurobiol. 2008 Nov 30;163(1-3):121-7
pubmed: 18565805
Cell. 2020 Jul 23;182(2):429-446.e14
pubmed: 32526206
J Comput Chem. 2005 Dec;26(16):1781-802
pubmed: 16222654
Sci Adv. 2021 Jan 1;7(1):
pubmed: 33277323
Int J Mol Sci. 2021 Jul 24;22(15):
pubmed: 34360686
J Phys Chem B. 1998 Apr 30;102(18):3586-616
pubmed: 24889800
Science. 2020 May 8;368(6491):630-633
pubmed: 32245784
Virology. 2021 Nov;563:134-145
pubmed: 34536797
Cell Host Microbe. 2020 Sep 9;28(3):445-454.e6
pubmed: 32585135
Science. 2018 Oct 5;362(6410):75-79
pubmed: 30287659
Nature. 2020 May;581(7807):221-224
pubmed: 32225175
PLoS Biol. 2021 Dec 21;19(12):e3001065
pubmed: 34932557
Cell Host Microbe. 2021 Mar 10;29(3):477-488.e4
pubmed: 33535027
mBio. 2020 Oct 16;11(5):
pubmed: 33067385
Nat Commun. 2021 Aug 9;12(1):4886
pubmed: 34373458
Science. 2021 May 21;372(6544):815-821
pubmed: 33853970
Clin Otolaryngol Allied Sci. 2002 Jun;27(3):135-9
pubmed: 12071984
Nature. 2021 Aug;596(7871):276-280
pubmed: 34237773
Cell. 2021 Mar 4;184(5):1171-1187.e20
pubmed: 33621484
PLoS One. 2013 Aug 30;8(8):e72942
pubmed: 24023659
J Med Virol. 2021 Sep;93(9):5358-5366
pubmed: 33913555
Genome Res. 2004 Jun;14(6):1188-90
pubmed: 15173120
Nature. 2020 Dec;588(7837):327-330
pubmed: 32942285
Cell Host Microbe. 2021 Mar 10;29(3):322-324
pubmed: 33705702
J Virol. 2020 Dec 11;95(5):
pubmed: 33310888
Euro Surveill. 2021 Jan;26(1):
pubmed: 33413740
J Appl Physiol (1985). 1999 Oct;87(4):1260-5
pubmed: 10517750
Cell. 2021 May 13;184(10):2587-2594.e7
pubmed: 33861950
Cell Rep Med. 2020 Oct 20;1(7):100126
pubmed: 33015650
Elife. 2021 Feb 23;10:
pubmed: 33620031
PLoS One. 2020 Sep 18;15(9):e0238339
pubmed: 32946453
Cell Rep. 2021 Jul 13;36(2):109353
pubmed: 34237283
Cell. 2020 Oct 29;183(3):739-751.e8
pubmed: 32991842
Immunity. 2021 Sep 14;54(9):2143-2158.e15
pubmed: 34453881
Sci Total Environ. 2020 Jul 10;725:138436
pubmed: 32298883
Science. 2021 Feb 12;371(6530):741-745
pubmed: 33436525
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
Nature. 2021 Nov;599(7883):114-119
pubmed: 34488225
Cell Host Microbe. 2020 Dec 9;28(6):867-879.e5
pubmed: 33271067
PLoS Biol. 2021 Mar 29;19(3):e3001158
pubmed: 33780434
Nat Struct Mol Biol. 2012 Sep;19(9):893-9
pubmed: 22864288
Nature. 2021 May;593(7858):266-269
pubmed: 33767447
Nature. 2020 May;581(7807):215-220
pubmed: 32225176
Nat Med. 2020 May;26(5):681-687
pubmed: 32327758
Science. 2020 Dec 18;370(6523):1464-1468
pubmed: 33184236
Cell. 2019 Feb 21;176(5):1026-1039.e15
pubmed: 30712865
Signal Transduct Target Ther. 2020 Jun 12;5(1):92
pubmed: 32532959
Sci Total Environ. 2020 Sep 20;736:139487
pubmed: 32479958
Nat Commun. 2021 Feb 8;12(1):848
pubmed: 33558493
Cell Host Microbe. 2021 Jul 14;29(7):1137-1150.e6
pubmed: 34133950