Genotyping of polyploid plants using quantitative PCR: application in the breeding of white-fleshed triploid loquats (Eriobotrya japonica).
Allele dosage
Flesh color
Loquat
Polyploid
Polyploid breeding
qPCR genotyping
Journal
Plant methods
ISSN: 1746-4811
Titre abrégé: Plant Methods
Pays: England
ID NLM: 101245798
Informations de publication
Date de publication:
03 Sep 2021
03 Sep 2021
Historique:
received:
29
04
2021
accepted:
24
08
2021
entrez:
4
9
2021
pubmed:
5
9
2021
medline:
5
9
2021
Statut:
epublish
Résumé
Ploidy manipulation is effective in seedless loquat breeding, in which flesh color is a key agronomic and economic trait. Few techniques are currently available for detecting the genotypes of polyploids in plants, but this ability is essential for most genetic research and molecular breeding. We developed a system for genotyping by quantitative PCR (qPCR) that allowed flesh color genotyping in multiple tetraploid and triploid loquat varieties (lines). The analysis of 13 different ratios of DNA mixtures between two homozygous diploids (AA and aa) showed that the proportion of allele A has a high correlation (R qPCR can detect the flesh color genotypes of loquat polyploids and provides an alternative method for analyzing polyploid genotype and breeding, dose effects and allele-specific expression.
Sections du résumé
BACKGROUND
BACKGROUND
Ploidy manipulation is effective in seedless loquat breeding, in which flesh color is a key agronomic and economic trait. Few techniques are currently available for detecting the genotypes of polyploids in plants, but this ability is essential for most genetic research and molecular breeding.
RESULTS
RESULTS
We developed a system for genotyping by quantitative PCR (qPCR) that allowed flesh color genotyping in multiple tetraploid and triploid loquat varieties (lines). The analysis of 13 different ratios of DNA mixtures between two homozygous diploids (AA and aa) showed that the proportion of allele A has a high correlation (R
CONCLUSIONS
CONCLUSIONS
qPCR can detect the flesh color genotypes of loquat polyploids and provides an alternative method for analyzing polyploid genotype and breeding, dose effects and allele-specific expression.
Identifiants
pubmed: 34479588
doi: 10.1186/s13007-021-00792-9
pii: 10.1186/s13007-021-00792-9
pmc: PMC8418031
doi:
Types de publication
Journal Article
Langues
eng
Pagination
93Subventions
Organisme : National Natural Science Foundation of China (NSFC)
ID : 31701876
Organisme : Innovation Research Group Funds for Chongqing Universities
ID : CXQT19005
Organisme : Fundamental Research Funds for the Central Universities
ID : XDJK2019AA001
Organisme : Chongqing Science & Technology Bureau
ID : 2018jscx-mszdX0054
Informations de copyright
© 2021. The Author(s).
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