Designing microfluidic devices for behavioral screening of multiple zebrafish larvae.
behavioral screening
electrical stimulation
microfluidics
multi-fish screening
zebrafish
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
revised:
25
08
2021
received:
07
02
2021
accepted:
30
08
2021
pubmed:
5
9
2021
medline:
19
1
2022
entrez:
4
9
2021
Statut:
ppublish
Résumé
Microfluidic devices are being used for phenotypic screening of zebrafish larvae in fundamental and pre-clinical research. A challenge for the broad use of these microfluidic devices is their low throughput, especially in behavioral assays. Previously, we introduced the tail locomotion of a semi-mobile zebrafish larva evoked on-demand with electric signal in a microfluidic device. Here, we report the lessons learned for increasing the number of specimens from one to four larvae in this device. Multiple parameters including loading and testing time per fish and loading and orientation efficiencies were refined to optimize the performance of modified designs. Flow and electric field simulations within the final device provided insight into the flow behavior and functionality of traps when compared to previous single-larva devices. Outcomes led to a new design which decreased the testing time per larva by ≈60%. Further, loading and orientation efficiencies increased by more than 80%. Critical behavioral parameters such as response duration and tail beat frequency were similar in both single and quadruple-fish devices. The developed microfluidic device has significant advantages for greater throughput and efficiency when behavioral phenotyping is required in various applications, including chemical testing in toxicology and gene screening.
Sections du résumé
BACKGROUND
BACKGROUND
Microfluidic devices are being used for phenotypic screening of zebrafish larvae in fundamental and pre-clinical research. A challenge for the broad use of these microfluidic devices is their low throughput, especially in behavioral assays. Previously, we introduced the tail locomotion of a semi-mobile zebrafish larva evoked on-demand with electric signal in a microfluidic device. Here, we report the lessons learned for increasing the number of specimens from one to four larvae in this device.
METHODS AND RESULTS
RESULTS
Multiple parameters including loading and testing time per fish and loading and orientation efficiencies were refined to optimize the performance of modified designs. Flow and electric field simulations within the final device provided insight into the flow behavior and functionality of traps when compared to previous single-larva devices. Outcomes led to a new design which decreased the testing time per larva by ≈60%. Further, loading and orientation efficiencies increased by more than 80%. Critical behavioral parameters such as response duration and tail beat frequency were similar in both single and quadruple-fish devices.
CONCLUSION
CONCLUSIONS
The developed microfluidic device has significant advantages for greater throughput and efficiency when behavioral phenotyping is required in various applications, including chemical testing in toxicology and gene screening.
Identifiants
pubmed: 34480402
doi: 10.1002/biot.202100076
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2100076Subventions
Organisme : Ontario Ministry of Colleges and Universities
Organisme : Natural Sciences and Engineering Research Council of Canada
Organisme : Ontario Trillium Foundation
Informations de copyright
© 2021 Wiley-VCH GmbH.
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