Metabolic imaging of human cumulus cells reveals associations among metabolic profiles of cumulus cells, patient clinical factors, and oocyte maturity.
Cumulus cells
cumulus-oocyte complexes
fluorescence lifetime imaging microscopy
human oocytes
maturity
metabolism
Journal
Fertility and sterility
ISSN: 1556-5653
Titre abrégé: Fertil Steril
Pays: United States
ID NLM: 0372772
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
received:
15
04
2021
revised:
27
07
2021
accepted:
29
07
2021
pubmed:
6
9
2021
medline:
22
12
2021
entrez:
5
9
2021
Statut:
ppublish
Résumé
To determine whether fluorescence lifetime imaging microscopy (FLIM) detects differences in metabolic state among cumulus cell samples and whether their metabolic state is associated with patient age, body mass index (BMI), and antimüllerian hormone (AMH) level and maturity of the oocyte. Prospective observational study. Academic laboratory. Cumulus cell (CC) clusters from cumulus-oocyte complexes were collected from patients undergoing assisted reproductive technology treatment after oocyte retrieval and vitrified. Cumulus cell metabolism was assessed using FLIM to measure autofluorescence of nicotinamide adenine (phosphate) dinucleotide and flavine adenine dinucleotide, endogenous coenzymes essential for cellular respiration and glycolysis. Patient age, BMI, and AMH level and the maturity of the corresponding oocytes were recorded. Quantitative information from FLIM was obtained regarding metabolite concentrations from fluorescence intensity and metabolite enzyme engagement from fluorescence lifetimes. Associations were investigated between each FLIM parameter and oocyte maturity and patient age, BMI, and AMH. Variance between CC clusters within and between patients was determined. Of 619 CC clusters from 193 patients, 90 were associated with immature oocytes and 505 with metaphase II oocytes. FLIM enabled quantitative measurements of the metabolic state of CC clusters. These parameters were significantly correlated with patient age and AMH independently, but not with BMI. Cumulus cell nicotinamide adenine (phosphate) dinucleotide FLIM parameters and redox ratio were significantly associated with maturity of the enclosed oocyte. FLIM detects variations in the metabolic state of CCs, showing a greater variance among clusters from each patient than between patients. Fluorescence lifetime imaging microscopy can detect CC metabolic associations with patient age and AMH and variations between mature and immature oocytes, suggesting the potential utility of this technique to help identify superior oocytes.
Identifiants
pubmed: 34481639
pii: S0015-0282(21)01808-2
doi: 10.1016/j.fertnstert.2021.07.1204
pmc: PMC8627436
mid: NIHMS1732100
pii:
doi:
Types de publication
Journal Article
Observational Study
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1651-1662Subventions
Organisme : NICHD NIH HHS
ID : R01 HD092550
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Published by Elsevier Inc.
Références
Hum Reprod. 2020 May 1;35(5):1120-1129
pubmed: 32358599
Physiol Res. 2020 Feb 19;69(1):33-48
pubmed: 31854191
Development. 2004 Jul;131(13):3057-67
pubmed: 15163630
Mol Reprod Dev. 2002 May;62(1):113-23
pubmed: 11933168
Biol Reprod. 1991 Dec;45(6):851-9
pubmed: 1666851
Mol Hum Reprod. 2008 Dec;14(12):711-9
pubmed: 19028806
J Assist Reprod Genet. 2016 Mar;33(3):367-371
pubmed: 26749386
Front Endocrinol (Lausanne). 2018 Jun 29;9:327
pubmed: 30008696
Bioessays. 1991 Nov;13(11):569-74
pubmed: 1772412
Reproduction. 2003 Jul;126(1):27-34
pubmed: 12814344
Anal Biochem. 1995 Jul 1;228(2):312-7
pubmed: 8572312
Hum Reprod. 2017 Mar 1;32(3):607-614
pubmed: 28077604
Fertil Steril. 2012 Dec;98(6):1574-80.e5
pubmed: 22968048
Mol Endocrinol. 2014 Jun;28(6):790-804
pubmed: 24678733
Nat Methods. 2019 Dec;16(12):1226-1232
pubmed: 31570887
Fertil Steril. 2015 Feb;103(2):303-16
pubmed: 25497448
Hum Reprod Update. 2008 Mar-Apr;14(2):159-77
pubmed: 18175787
Biomark Med. 2010 Apr;4(2):241-63
pubmed: 20406068
Reproduction. 2001 May;121(5):647-53
pubmed: 11427152
J Clin Endocrinol Metab. 2016 May;101(5):2235-45
pubmed: 27003307
Biol Reprod. 2016 Dec;95(6):129
pubmed: 27683265
Mol Hum Reprod. 2010 Oct;16(10):715-25
pubmed: 20435609
Hum Reprod. 2017 Oct 1;32(10):2101-2109
pubmed: 28938736
J Cell Biol. 1976 Nov;71(2):680-6
pubmed: 825522
Trends Endocrinol Metab. 2012 Sep;23(9):420-8
pubmed: 22819213
Hum Reprod Update. 2016 Nov;22(6):725-743
pubmed: 27562289
J Assist Reprod Genet. 2018 Oct;35(10):1821-1830
pubmed: 30094760
Fertil Steril. 2011 Jul;96(1):47-52.e2
pubmed: 21575950
Fertil Steril. 2015 Feb;103(2):317-22
pubmed: 25639967
Reproduction. 2001 Dec;122(6):829-38
pubmed: 11732978
J Anim Sci. 2007 Mar;85(13 Suppl):E1-3
pubmed: 17322120
Aging (Albany NY). 2020 Feb 25;12(4):3950-3961
pubmed: 32096767
Mol Hum Reprod. 2006 Nov;12(11):655-60
pubmed: 17005595
J Microsc. 2012 Aug;247(2):119-36
pubmed: 22621335
BBA Clin. 2017 Apr 05;7:141-146
pubmed: 28660134
Fertil Steril. 2002 Nov;78(5):1046-8
pubmed: 12413991
J Assist Reprod Genet. 2019 Feb;36(2):223-228
pubmed: 30362054
Hum Reprod. 2015 Jul;30(7):1653-64
pubmed: 25994667
Hum Reprod. 2004 Dec;19(12):2869-74
pubmed: 15471935
Fertil Steril. 2013 Mar 15;99(4):979-97
pubmed: 23498999