Activation of the Thiazide-Sensitive Sodium-Chloride Cotransporter by Beta3-Adrenoreceptor in the Distal Convoluted Tubule.
beta3-adrenoreceptor
distal convoluted tubule
sodium-chloride cotransporter
sympathetic regulation
thiazide-sensitive Na-Cl cotransporter
Journal
Frontiers in physiology
ISSN: 1664-042X
Titre abrégé: Front Physiol
Pays: Switzerland
ID NLM: 101549006
Informations de publication
Date de publication:
2021
2021
Historique:
received:
15
04
2021
accepted:
16
07
2021
entrez:
6
9
2021
pubmed:
7
9
2021
medline:
7
9
2021
Statut:
epublish
Résumé
We previously showed that the beta-3 adrenergic receptor (BAR3) is expressed in most segments of the nephron where its agonism promotes a potent antidiuretic effect. We localized BAR3 in distal convoluted tubule (DCT) cells expressing the thiazide-sensitive sodium-chloride cotransporter (NCC). Aim of this study is to investigate the possible functional role of BAR3 on NCC modulation in DCT cells. Here, we found that, in mice, the knockout of BAR3 was paralleled by a significant attenuation of NCC phosphorylation, paralleled by reduced expression and activation of STE-20/SPS1-related proline-alanine-rich kinase (SPAK) and WNKs the main kinases involved in NCC activation. Conversely, in BAR1/2 knockout mice, we found reduced NCC abundance with no changes in the phosphorylation state of NCC. Moreover, selective BAR3 agonism promotes both SPAK and NCC activation in wild-type mouse kidney slices. In conclusion, our findings suggest a novel role for BAR3 in the regulation of NCC in DCT.
Identifiants
pubmed: 34483955
doi: 10.3389/fphys.2021.695824
pmc: PMC8414899
doi:
Types de publication
Journal Article
Langues
eng
Pagination
695824Informations de copyright
Copyright © 2021 Milano, Carmosino, Gerbino, Saponara, Lapi, Dal Monte, Bagnoli, Svelto and Procino.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Eur J Pediatr. 2012 Oct;171(10):1433-9
pubmed: 21404100
Annu Rev Physiol. 2016;78:367-89
pubmed: 26863326
Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):E879-E886
pubmed: 28096417
Am J Physiol Renal Physiol. 2016 Jan 1;310(1):F10-4
pubmed: 26511649
Kidney Int. 2001 Feb;59(2):515-31
pubmed: 11168934
J Cell Sci. 2008 Oct 15;121(Pt 20):3293-304
pubmed: 18843116
Lancet. 2009 Apr 11;373(9671):1275-81
pubmed: 19332353
J Biol Chem. 1999 Jun 11;274(24):16701-8
pubmed: 10358009
J Biol Chem. 2005 Dec 30;280(52):42685-93
pubmed: 16263722
Biochem Biophys Res Commun. 1999 Aug 11;261(3):870-6
pubmed: 10441518
Kidney Int. 2021 Aug;100(2):321-335
pubmed: 33940111
Pediatr Nephrol. 2007 Mar;22(3):326-32
pubmed: 17061123
Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):15556-61
pubmed: 25313067
J Physiol. 2001 Feb 15;531(Pt 1):105-15
pubmed: 11179395
Cell Physiol Biochem. 2018;48(2):847-862
pubmed: 30032151
J Am Soc Nephrol. 2017 Dec;28(12):3504-3517
pubmed: 28774999
Hypertension. 2014 Jul;64(1):178-84
pubmed: 24799612
J Mol Cell Cardiol. 2009 Nov;47(5):576-85
pubmed: 19766235
Front Med (Lausanne). 2018 Mar 29;5:82
pubmed: 29651418
N Engl J Med. 2009 Aug 27;361(9):932-4
pubmed: 19710497
Am J Physiol Renal Physiol. 2015 Apr 15;308(8):F799-808
pubmed: 25587121
Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4384-9
pubmed: 19240212
Kidney Int. 2010 Jul;78(2):160-9
pubmed: 20445498
Am J Physiol Renal Physiol. 2010 Mar;298(3):F502-9
pubmed: 20007345
J Biol Chem. 2006 Sep 29;281(39):28755-63
pubmed: 16887815
Biochem J. 2005 Oct 1;391(Pt 1):17-24
pubmed: 16083423
Nat Med. 2011 May;17(5):573-80
pubmed: 21499270
J Appl Physiol (1985). 2004 Jul;97(1):369-76
pubmed: 15047670
Pflugers Arch. 2014 Jan;466(1):107-18
pubmed: 24310820
J Clin Invest. 2014 Jun;124(6):2341-7
pubmed: 24892708
J Cell Sci. 2008 Mar 1;121(Pt 5):675-84
pubmed: 18270262
J Am Soc Nephrol. 2013 Feb;24(3):407-18
pubmed: 23393317
Hypertens Res. 2004 Nov;27(11):821-33
pubmed: 15824464
Kidney Int. 2014 Jul;86(1):127-38
pubmed: 24522493
Pharmacol Res. 2013 Dec;78:1-9
pubmed: 24083950
Am J Physiol Heart Circ Physiol. 2006 Jan;290(1):H192-9
pubmed: 16113068
Am J Physiol Renal Physiol. 2012 Mar 1;302(5):F533-9
pubmed: 22114204
Pflugers Arch. 2015 Nov;467(11):2235-41
pubmed: 25904388
Kidney Int. 2016 Sep;90(3):555-67
pubmed: 27206969
Am J Physiol Heart Circ Physiol. 2005 Dec;289(6):H2441-9
pubmed: 16040722