Contaminants and Where to Find Them: Microbiological Quality Control in Axenic Animal Facilities.
contaminants
culture
germ-free
microscopy
quantitative PCR
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2021
2021
Historique:
received:
13
05
2021
accepted:
26
07
2021
entrez:
6
9
2021
pubmed:
7
9
2021
medline:
7
9
2021
Statut:
epublish
Résumé
The use of axenic animal models in experimental research has exponentially grown in the past few years and the most reliable way for confirming their axenic status remains unclear. It is especially the case when using individual ventilated positive-pressure cages such as the Isocage. This type of cage are at a greater risk of contamination and expose animals to a longer handling process leading to more potential stress when opened compared to isolators. The aim of this study was to propose simple ways to detect microbial contaminants with Isocages type isolator resulting by developing, validating and optimizing three different methods (culture, microscopy, and molecular). These three approaches were also tested
Identifiants
pubmed: 34484147
doi: 10.3389/fmicb.2021.709399
pmc: PMC8415547
doi:
Types de publication
Journal Article
Langues
eng
Pagination
709399Informations de copyright
Copyright © 2021 Lebeuf, Turgeon, Faubert, Pleau, Robillard, Paradis, Marette and Duchaine.
Déclaration de conflit d'intérêts
AM research chair was given in partnership between IRSC and Pfizer Canada and he is consequently not employed by Pfizer Canada. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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