Effect of TNF-α on osteocyte RANKL expression during orthodontic tooth movement.
Orthodontic tooth movement
Osteoclast
Osteocyte
RANKL
TNF-α
Journal
Journal of dental sciences
ISSN: 2213-8862
Titre abrégé: J Dent Sci
Pays: Netherlands
ID NLM: 101293181
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
16
02
2021
revised:
11
03
2021
entrez:
6
9
2021
pubmed:
7
9
2021
medline:
7
9
2021
Statut:
ppublish
Résumé
Orthodontic tooth movement (OTM) is facilitated by two events; bone resorption on the compression side and bone formation on the tension side simultaneously termed bone remodeling. Osteocytes play a critical role in bone remodeling during OTM, as they have been described as the critical source of nuclear factor-κB ligand (RANKL) necessary for bone remodeling during OTM. Tumor necrosis factor (TNF)-α is a cytokine that acts by amplifying RANKL expression in osteocytes. In this study, we evaluated the effects of TNF-α on RANKL expression in osteocyte during OTM. We assessed whether TNF-α influenced RANKL expression in osteocyte during orthodontic tooth movement by using wild-type (WT) and TNF receptor I and II deficient (TNFRsKO) mice. A Nickel-titanium closed coil spring was attached to the maxillary alveolar bone near the incisors and the upper left first molar, and the first molars were moved mesially in WT and TNFRsKO mice. After OTM, the number of RANKL-positive osteocytes in the alveolar bone was evaluated by immunohistochemistry. The number of RANKL-positive osteocyte in the alveolar bone significantly increased in WT mice than in TNFRsKO mice after OTM. The results indicate that TNF-α induces the expression of RANKL in osteocyte during OTM.
Sections du résumé
BACKGROUND/PURPOSE
OBJECTIVE
Orthodontic tooth movement (OTM) is facilitated by two events; bone resorption on the compression side and bone formation on the tension side simultaneously termed bone remodeling. Osteocytes play a critical role in bone remodeling during OTM, as they have been described as the critical source of nuclear factor-κB ligand (RANKL) necessary for bone remodeling during OTM. Tumor necrosis factor (TNF)-α is a cytokine that acts by amplifying RANKL expression in osteocytes. In this study, we evaluated the effects of TNF-α on RANKL expression in osteocyte during OTM.
MATERIALS AND METHODS
METHODS
We assessed whether TNF-α influenced RANKL expression in osteocyte during orthodontic tooth movement by using wild-type (WT) and TNF receptor I and II deficient (TNFRsKO) mice. A Nickel-titanium closed coil spring was attached to the maxillary alveolar bone near the incisors and the upper left first molar, and the first molars were moved mesially in WT and TNFRsKO mice. After OTM, the number of RANKL-positive osteocytes in the alveolar bone was evaluated by immunohistochemistry.
RESULTS
RESULTS
The number of RANKL-positive osteocyte in the alveolar bone significantly increased in WT mice than in TNFRsKO mice after OTM.
CONCLUSION
CONCLUSIONS
The results indicate that TNF-α induces the expression of RANKL in osteocyte during OTM.
Identifiants
pubmed: 34484587
doi: 10.1016/j.jds.2021.03.006
pii: S1991-7902(21)00048-9
pmc: PMC8403810
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1191-1197Informations de copyright
© 2021 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.
Déclaration de conflit d'intérêts
The authors have no conflicts of interest relevant to this article.
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