Strong correlation between urine and vaginal swab samples for bacterial vaginosis.

BV G. vaginalis South Africa bacterial vaginosis ddPCR pregnant women swab urine

Journal

Southern African journal of infectious diseases
ISSN: 2313-1810
Titre abrégé: S Afr J Infect Dis
Pays: South Africa
ID NLM: 101646666

Informations de publication

Date de publication:
2021
Historique:
received: 24 03 2020
accepted: 15 03 2021
entrez: 6 9 2021
pubmed: 7 9 2021
medline: 7 9 2021
Statut: epublish

Résumé

Vaginal swabs have been traditionally used for the diagnosis of bacterial vaginosis (BV). Currently, there are limited studies that have investigated the use of other sample types other than vaginal swabs for the detection of BV from South African populations. This study investigated whether urine can be used for the detection of BV-associated microorganisms in South African pregnant women. One-hundred self-collected vaginal swabs and urine samples were obtained from women presenting for antenatal care at King Edward VIII Hospital in Durban. The BD MAX™ vaginal panel assay was used for diagnosing BV and droplet digital polymerase chain reaction was used to quantify Median copy numbers obtained for This study highlights the appropriateness of urine for the detection of microorganisms associated with BV.

Sections du résumé

BACKGROUND BACKGROUND
Vaginal swabs have been traditionally used for the diagnosis of bacterial vaginosis (BV). Currently, there are limited studies that have investigated the use of other sample types other than vaginal swabs for the detection of BV from South African populations. This study investigated whether urine can be used for the detection of BV-associated microorganisms in South African pregnant women.
METHODS METHODS
One-hundred self-collected vaginal swabs and urine samples were obtained from women presenting for antenatal care at King Edward VIII Hospital in Durban. The BD MAX™ vaginal panel assay was used for diagnosing BV and droplet digital polymerase chain reaction was used to quantify
RESULTS RESULTS
Median copy numbers obtained for
CONCLUSION CONCLUSIONS
This study highlights the appropriateness of urine for the detection of microorganisms associated with BV.

Identifiants

pubmed: 34485489
doi: 10.4102/sajid.v36i1.199
pii: SAJID-36-199
pmc: PMC8377811
doi:

Types de publication

Journal Article

Langues

eng

Pagination

199

Informations de copyright

© 2021. The Authors.

Déclaration de conflit d'intérêts

The authors declare that they have no financial or personal relationships that may have inappropriately influenced them in writing this article.

Références

BJOG. 2017 Jan;124(1):61-69
pubmed: 27396541
PLoS One. 2013 Oct 04;8(10):e76589
pubmed: 24124575
Trends Microbiol. 2017 Mar;25(3):182-191
pubmed: 27914761
J Clin Microbiol. 1991 Feb;29(2):297-301
pubmed: 1706728
Am J Obstet Gynecol. 2007 Nov;197(5):477.e1-8
pubmed: 17980180
Indian J Med Res. 2015 Jun;141(6):799-806
pubmed: 26205023
J Acquir Immune Defic Syndr Hum Retrovirol. 1999 Jan 1;20(1):52-9
pubmed: 9928730
FEMS Immunol Med Microbiol. 2002 Dec 13;34(4):277-81
pubmed: 12443827
J Infect Dis. 1999 Dec;180(6):1863-8
pubmed: 10558942
J Clin Microbiol. 2014 Jan;52(1):218-25
pubmed: 24197876
PLoS One. 2013;8(1):e53997
pubmed: 23320114
J Infect Dis. 2005 Oct 15;192(8):1372-80
pubmed: 16170754
PeerJ. 2015 Feb 17;3:e780
pubmed: 25737820
BMC Infect Dis. 2017 Jun 5;17(1):394
pubmed: 28583109
PLoS One. 2017 Feb 27;12(2):e0172522
pubmed: 28241058
J Infect Dis. 2006 Sep 15;194(6):828-36
pubmed: 16941351
Am J Obstet Gynecol. 2013 Dec;209(6):505-23
pubmed: 23659989
Clin Infect Dis. 2003 Aug 1;37(3):319-25
pubmed: 12884154
Clin Microbiol Rev. 2016 Apr;29(2):223-38
pubmed: 26864580
Iran J Microbiol. 2014 Dec;6(6):409-14
pubmed: 25926959
PLoS One. 2013 Apr 09;8(4):e60670
pubmed: 23585843
Biomed Res Int. 2015;2015:645853
pubmed: 26078959
Microbiology (Reading). 2016 Mar;162(3):466-475
pubmed: 26747455
Sex Transm Infect. 2016 Nov;92(7):487-491
pubmed: 26965870
Int J Microbiol. 2017;2017:4919404
pubmed: 28831285
Int J STD AIDS. 2020 Mar;31(4):303-311
pubmed: 32050856
AIDS. 1995 Sep;9(9):1093-7
pubmed: 8527084

Auteurs

Deshanta Naicker (D)

School of Clinical Medicine Research Laboratory, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa.

Veron Ramsuran (V)

KwaZulu-Natal Research and Innovation Sequencing Platform (KRISP), School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa.
Centre for the AIDS Programme of Research in South Africa (CAPRISA), University of KwaZulu-Natal, Durban, South Africa.

Meleshni Naicker (M)

School of Clinical Medicine Research Laboratory, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa.

Fazana Dessai (F)

School of Clinical Medicine Research Laboratory, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa.

Jennifer Giandhari (J)

KwaZulu-Natal Research and Innovation Sequencing Platform (KRISP), School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa.

Partson Tinarwo (P)

Department of Biostatistics, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa.

Nathlee Abbai (N)

School of Clinical Medicine Research Laboratory, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa.

Classifications MeSH