LATE-a novel sensitive cell-based assay for the study of CRISPR/Cas9-related long-term adverse treatment effects.
CRISPR/Cas
LeGO vectors
cellular assay
gene therapy
genome editing
high-fidelity Cas9
off-targets
side effects
Journal
Molecular therapy. Methods & clinical development
ISSN: 2329-0501
Titre abrégé: Mol Ther Methods Clin Dev
Pays: United States
ID NLM: 101624857
Informations de publication
Date de publication:
10 Sep 2021
10 Sep 2021
Historique:
received:
28
04
2020
accepted:
23
07
2021
entrez:
6
9
2021
pubmed:
7
9
2021
medline:
7
9
2021
Statut:
epublish
Résumé
Since the introduction of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), genome editing has been broadly applied in basic research and applied biotechnology, whereas translation into clinical testing has raised safety concerns. Indeed, although frequencies and locations of off-target events have been widely addressed, little is known about their potential biological consequences in large-scale long-term settings. We have developed a long-term adverse treatment effect (LATE)
Identifiants
pubmed: 34485609
doi: 10.1016/j.omtm.2021.07.004
pii: S2329-0501(21)00121-2
pmc: PMC8399046
doi:
Types de publication
Journal Article
Langues
eng
Pagination
249-262Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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