Coherent confocal light scattering spectroscopic microscopy evaluates cancer progression and aggressiveness in live cells and tissue.
cancer
coherence
confocal microscopy
light scattering
spectroscopy
Journal
ACS photonics
ISSN: 2330-4022
Titre abrégé: ACS Photonics
Pays: United States
ID NLM: 101634366
Informations de publication
Date de publication:
21 Jul 2021
21 Jul 2021
Historique:
entrez:
6
9
2021
pubmed:
7
9
2021
medline:
7
9
2021
Statut:
ppublish
Résumé
The observation of biological structures in live cells beyond the diffraction limit with super-resolution fluorescence microscopy is limited by the ability of fluorescence probes to permeate live cells and the effect of these probes, which are often toxic, on cellular behavior. Here we present a coherent confocal light scattering and absorption spectroscopic microscopy that for the first time enables the use of large numerical aperture optics to characterize structures in live cells down to 10 nm spatial scales, well beyond the diffraction limit. Not only does this new capability allow high resolution microscopy with light scattering contrast, but it can also be used with almost any light scattering spectroscopic application which employs lenses. We demonstrate that the coherent light scattering contrast based technique allows continuous temporal tracking of the transition from non-cancerous to an early cancerous state in live cells, without exogenous markers. We also use the technique to sense differences in the aggressiveness of cancer in live cells and for label free identification of different grades of cancer in resected tumor tissues.
Identifiants
pubmed: 34485615
doi: 10.1021/acsphotonics.1c00217
pmc: PMC8411902
mid: NIHMS1714864
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2050-2059Subventions
Organisme : NCI NIH HHS
ID : R01 CA205431
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA218382
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB003472
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB025173
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA228029
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
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