Adjunctive dental therapies in caries-active children: Shifting the cariogenic salivary microbiome from dysbiosis towards non-cariogenic health.
Caries-active children
Dental caries
Dysbiosis
Microbiome shift
Oral health
Povidone iodine
Salivary microbiome
Stentrophomonas maltophila
Journal
Human microbiome journal
ISSN: 2452-2317
Titre abrégé: Hum Microb J
Pays: England
ID NLM: 101729981
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
entrez:
6
9
2021
pubmed:
7
9
2021
medline:
7
9
2021
Statut:
ppublish
Résumé
The oral microbiome is a complex assembly of microbial species, whose constituents can tilt the balance towards progression of oral disease or sustained health. Recently we identified sex-specific differences in the salivary microbiome contained within caries-active and caries-free children. In this study, we sought to ascertain if adjunctive dental therapies, including povidone iodine and chlorhexidine, were effective in shifting the cariogenic microbiome from dysbiosis to non-cariogenic health. We recruited young children (ages 2-12 years) to enter five enrollment groups, with each group (N = 9-30 participants/group) receiving caries restorative and/or adjunctive therapies, either singularly or in combination (OHSU IRB #6535). Saliva specimens were collected pre- and post-treatment (4-8 weeks) of caries preventive measures, and oral microbiota were identified using next generation sequencing (HOMI With the use of multi-dimensional scaling plots, support vector machine learning, odds ratio analysis, and other statistical methods, we have determined that treatment with povidone iodine can shift the composition of the salivary cariogenic microbiome to include higher proportions of aerobic microorganisms, such as We have identified microorganisms that are associated with caries-active children and have determined that povidone iodine is an effective adjunctive therapy that has the potential to shift the composition of the cariogenic microbiome to one more closely aligned with non-cariogenic health.
Sections du résumé
BACKGROUND
BACKGROUND
The oral microbiome is a complex assembly of microbial species, whose constituents can tilt the balance towards progression of oral disease or sustained health. Recently we identified sex-specific differences in the salivary microbiome contained within caries-active and caries-free children. In this study, we sought to ascertain if adjunctive dental therapies, including povidone iodine and chlorhexidine, were effective in shifting the cariogenic microbiome from dysbiosis to non-cariogenic health.
DESIGN
METHODS
We recruited young children (ages 2-12 years) to enter five enrollment groups, with each group (N = 9-30 participants/group) receiving caries restorative and/or adjunctive therapies, either singularly or in combination (OHSU IRB #6535). Saliva specimens were collected pre- and post-treatment (4-8 weeks) of caries preventive measures, and oral microbiota were identified using next generation sequencing (HOMI
RESULTS
RESULTS
With the use of multi-dimensional scaling plots, support vector machine learning, odds ratio analysis, and other statistical methods, we have determined that treatment with povidone iodine can shift the composition of the salivary cariogenic microbiome to include higher proportions of aerobic microorganisms, such as
CONCLUSION
CONCLUSIONS
We have identified microorganisms that are associated with caries-active children and have determined that povidone iodine is an effective adjunctive therapy that has the potential to shift the composition of the cariogenic microbiome to one more closely aligned with non-cariogenic health.
Identifiants
pubmed: 34485763
doi: 10.1016/j.humic.2020.100077
pmc: PMC8415465
mid: NIHMS1730542
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NIDCR NIH HHS
ID : R15 DE024317
Pays : United States
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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