CRISPR/Cas12a-mediated liposome-amplified strategy for the photoelectrochemical detection of nucleic acid.
Bacterial Proteins
/ chemistry
Biosensing Techniques
/ methods
CRISPR-Associated Proteins
/ chemistry
CRISPR-Cas Systems
DNA, Single-Stranded
/ chemistry
DNA, Viral
/ analysis
Dopamine
/ chemistry
Electrochemical Techniques
/ methods
Endodeoxyribonucleases
/ chemistry
Fluoresceins
/ chemistry
Fluorescent Dyes
/ chemistry
Human papillomavirus 16
/ chemistry
Liposomes
/ chemistry
Magnetic Phenomena
Photochemical Processes
Journal
Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838
Informations de publication
Date de publication:
06 Sep 2021
06 Sep 2021
Historique:
entrez:
6
9
2021
pubmed:
7
9
2021
medline:
13
10
2021
Statut:
epublish
Résumé
This study reports a photoelectrochemical biosensor for dopamine-loaded liposome-encoded magnetic beads cleaved by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas 12a system for the quantification of human papilloma virus (HPV)-related DNA using neodymium-doped BiOBr nanosheets (Nd-BiOBr) as a photoactive matrix. Magnetic beads and dopamine-loaded liposomes are covalently attached to the both ends of ssDNA to construct dumbbell-shaped dopamine-loaded liposome-encoded magnetic bead (DLL-MB) probes. When the guide RNA binds to the target HPV-16, the ssDNA will be cleaved by Cas12a, thereby degrading the double dumbbell probes. After magnetic separation, the dissolved DLLs are treated with Triton X-100 to release the dopamine (as an electron donor), which was then detected by an amplified photocurrent using the Nd-BiOBr-based photoelectrode.
Substances chimiques
Bacterial Proteins
0
CRISPR-Associated Proteins
0
DNA, Single-Stranded
0
DNA, Viral
0
Fluoresceins
0
Fluorescent Dyes
0
Liposomes
0
Cas12a protein
EC 3.1.-
Endodeoxyribonucleases
EC 3.1.-
Dopamine
VTD58H1Z2X
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM