Toxicological effects of silver nanoparticles and cadmium chloride in macrophage cell line (RAW 264.7): An in vitro approach.
Cadmium
Co-exposure
Interaction
Murine macrophages
Silver nanoparticles
Journal
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)
ISSN: 1878-3252
Titre abrégé: J Trace Elem Med Biol
Pays: Germany
ID NLM: 9508274
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
04
04
2021
revised:
19
08
2021
accepted:
31
08
2021
pubmed:
7
9
2021
medline:
17
2
2022
entrez:
6
9
2021
Statut:
ppublish
Résumé
Silver nanoparticles (AgNP) are largely used in nanotechnological products, but the real risks for human and environment are still poorly understood if we consider the effects of mixtures of AgNP and environmental contaminants, such as non-essential metals. The aim of the present study was to investigate the cytotoxicity and toxicological interaction of AgNP (1-4 nm, 0.36 and 3.6 μg mL Effects were observed after a few hours (4 h) on reactive oxygen species (ROS) and became more pronounced after 24 h-exposure. Cell death occurred by apoptosis, and loss of cell viability (24 h-exposure) was preceded by increases of ROS levels and DNA repair foci, but not of NO levels. Co-exposure potentiated some effects (decrease of cell viability and increase of ROS and NO levels), indicating toxicological interaction. These effects are important findings that must be better investigated, since the interaction of Cd with AgNP from nanoproducts may impair the function of macrophages and represent a health risk for humans.
Sections du résumé
BACKGROUND
BACKGROUND
Silver nanoparticles (AgNP) are largely used in nanotechnological products, but the real risks for human and environment are still poorly understood if we consider the effects of mixtures of AgNP and environmental contaminants, such as non-essential metals.
METHODS
METHODS
The aim of the present study was to investigate the cytotoxicity and toxicological interaction of AgNP (1-4 nm, 0.36 and 3.6 μg mL
RESULTS
RESULTS
Effects were observed after a few hours (4 h) on reactive oxygen species (ROS) and became more pronounced after 24 h-exposure. Cell death occurred by apoptosis, and loss of cell viability (24 h-exposure) was preceded by increases of ROS levels and DNA repair foci, but not of NO levels. Co-exposure potentiated some effects (decrease of cell viability and increase of ROS and NO levels), indicating toxicological interaction.
CONCLUSION
CONCLUSIONS
These effects are important findings that must be better investigated, since the interaction of Cd with AgNP from nanoproducts may impair the function of macrophages and represent a health risk for humans.
Identifiants
pubmed: 34488184
pii: S0946-672X(21)00144-9
doi: 10.1016/j.jtemb.2021.126854
pii:
doi:
Substances chimiques
Reactive Oxygen Species
0
Cadmium
00BH33GNGH
Silver
3M4G523W1G
Cadmium Chloride
J6K4F9V3BA
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126854Informations de copyright
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