Engineering Fluorophore Recycling in a Fluorogenic RNA Aptamer.
RNA imaging
fluorescent probes
fluorophore recycling
photostability
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
02 11 2021
02 11 2021
Historique:
revised:
27
07
2021
received:
23
06
2021
pubmed:
8
9
2021
medline:
16
12
2021
entrez:
7
9
2021
Statut:
ppublish
Résumé
Fluorogenic aptamers can potentially show minimal photobleaching during continuous irradiation since any photobleached fluorophore can exchange with fluorescent dyes in the media. However, fluorophores have not been designed to maximize "fluorophore recycling." Here we describe TBI, a novel fluorophore for the Broccoli fluorogenic aptamer. Previous fluorophores either fail to rapidly dissociate when they undergo photobleaching via cis-trans isomerization, or bind slowly, resulting in extended periods after dissociation of the photobleached fluorophore when no fluorophore is bound. By contrast, photobleached TBI dissociates rapidly from Broccoli, and TBI from the media rapidly replaces dissociated photobleached fluorophore. Using TBI, Broccoli exhibits markedly enhanced fluorescence in cells during continuous imaging. These data show that designing fluorophores to optimize fluorophore recycling can lead to enhanced fluorescence of fluorogenic aptamers.
Identifiants
pubmed: 34490956
doi: 10.1002/anie.202108338
pmc: PMC8661118
mid: NIHMS1739311
doi:
Substances chimiques
Aptamers, Nucleotide
0
Fluorescent Dyes
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
24153-24161Subventions
Organisme : NIH HHS
ID : NS111631
Pays : United States
Organisme : starr cancer consortium grant
ID : I12-0051
Organisme : NIH HHS
ID : NS111631
Pays : United States
Organisme : talent research start-up fund, beijing institutes of life science, chinese academy of sciences (cas)
Organisme : NINDS NIH HHS
ID : R35 NS111631
Pays : United States
Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
J Am Chem Soc. 2014 Jan 29;136(4):1198-201
pubmed: 24393009
J Am Chem Soc. 2019 Oct 2;141(39):15547-15556
pubmed: 31490678
Nat Biotechnol. 2019 Nov;37(11):1287-1293
pubmed: 31548726
ACS Cent Sci. 2020 Nov 25;6(11):1938-1954
pubmed: 33274271
Nat Chem Biol. 2017 Nov;13(11):1187-1194
pubmed: 28945233
Science. 2012 Mar 9;335(6073):1194
pubmed: 22403384
Nat Chem Biol. 2020 Jan;16(1):69-76
pubmed: 31636432
Angew Chem Int Ed Engl. 2020 Mar 9;59(11):4511-4518
pubmed: 31850609
J Med Chem. 2012 May 10;55(9):4489-500
pubmed: 22500930
Nat Struct Mol Biol. 2014 Aug;21(8):658-63
pubmed: 25026079
Annu Rev Biochem. 2016 Jun 2;85:349-73
pubmed: 27294440
Nat Methods. 2011 Nov 13;9(1):68-71
pubmed: 22081126
J Am Chem Soc. 2020 Aug 19;142(33):14117-14124
pubmed: 32698574
J Mol Biol. 2006 Oct 13;363(1):98-113
pubmed: 16962138
Nucleic Acids Res. 2018 Oct 12;46(18):e110
pubmed: 29931157
J Am Chem Soc. 2013 Dec 18;135(50):19033-8
pubmed: 24286188
Biochemistry. 2019 Mar 26;58(12):1560-1564
pubmed: 30838859
ACS Chem Biol. 2014 Oct 17;9(10):2412-20
pubmed: 25101481
Science. 2011 Jul 29;333(6042):642-6
pubmed: 21798953
Nat Biotechnol. 2020 Dec;38(12):1451-1459
pubmed: 32632301
Anal Chim Acta. 2021 Dec 15;1188:338859
pubmed: 34794573
Cell Chem Biol. 2021 Nov 18;28(11):1569-1580.e4
pubmed: 34010626
Curr Opin Chem Biol. 2020 Aug;57:177-183
pubmed: 32829251
Nat Biotechnol. 2019 Jun;37(6):667-675
pubmed: 30962542
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10437-42
pubmed: 9724721
Science. 2019 Feb 22;363(6429):884-887
pubmed: 30792304