Influence of caries activity and number of saliva donors: mineral and microbiological responses in a microcosm biofilm model.
Journal
Journal of applied oral science : revista FOB
ISSN: 1678-7765
Titre abrégé: J Appl Oral Sci
Pays: Brazil
ID NLM: 101189774
Informations de publication
Date de publication:
2021
2021
Historique:
received:
30
09
2020
accepted:
06
05
2021
entrez:
8
9
2021
pubmed:
9
9
2021
medline:
11
9
2021
Statut:
epublish
Résumé
this study evaluated the mineral and microbiological response of biofilms originating from different types of saliva inoculum with distinct levels of caries activity. the biofilms grown over enamel specimens originated from saliva collected from a single donor or five donors with two distinct levels of caries activity (caries-active and caries-free) or from pooling saliva from ten donors (five caries-active and five caries-free). The percentage surface hardness change (%SHC) and microbiological counts served as outcome variables. the caries activity of donors did not affect the %SHC values. Inoculum from five donors compared to a single donor showed higher %SHC values (p=0.019). Higher lactobacilli counts were observed when saliva from caries-active donors was used as the inoculum (p=0.017). Pooled saliva from both caries activity levels showed higher mutans streptococci counts (p<0.017). Overall, pooled saliva increased the mineral response of the derived biofilms, but all the inoculum conditions formed cariogenic biofilms and caries lesions independently of caries activity.
Identifiants
pubmed: 34495103
pii: S1678-77572021000100439
doi: 10.1590/1678-7757-2020-0778
pmc: PMC8425900
pii:
doi:
Substances chimiques
Minerals
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e20200778Références
Caries Res. 2000 Nov-Dec;34(6):491-7
pubmed: 11093024
Clin Oral Investig. 2014 Sep;18(7):1779-84
pubmed: 24317957
J Dent. 2005 Mar;33(3):193-207
pubmed: 15725520
Lancet. 2019 Jul 20;394(10194):249-260
pubmed: 31327369
J Dent Res. 2015 May;94(5):650-8
pubmed: 25740856
Dent Clin North Am. 2010 Jul;54(3):441-54
pubmed: 20630188
Biofouling. 2017 Sep;33(8):661-675
pubmed: 28792234
Lancet. 2007 Jan 6;369(9555):51-9
pubmed: 17208642
Lancet. 2019 Jul 20;394(10194):261-272
pubmed: 31327370
J Am Dent Assoc. 2009 Sep;140 Suppl 1:25S-34S
pubmed: 19723928
Caries Res. 2011;45(6):510-7
pubmed: 21967836
Biofouling. 2014 Sep;30(8):903-9
pubmed: 25184431
J Dent. 2020 May;96:103328
pubmed: 32240676
Biofouling. 2011 Oct;27(9):1057-63
pubmed: 22044385
BMC Oral Health. 2006 Jun 15;6 Suppl 1:S14
pubmed: 16934115
Caries Res. 2017;51(5):475-481
pubmed: 28858860
J Appl Microbiol. 2006 May;100(5):1123-31
pubmed: 16630013
Caries Res. 2016;50(2):97-103
pubmed: 26919718
Caries Res. 2006;40(2):130-5
pubmed: 16508270
Adv Appl Microbiol. 2009;69:99-132
pubmed: 19729092
J Oral Microbiol. 2015 Mar 03;7:26149
pubmed: 25740099
J Appl Microbiol. 2005;98(3):624-34
pubmed: 15715865
J Oral Sci. 2011 Sep;53(3):273-82
pubmed: 21959653
BMC Med Genomics. 2011 Mar 04;4:22
pubmed: 21371338
Arch Oral Biol. 2001 Jun;46(6):477-86
pubmed: 11311195